A study in Bodoquena found Physalaemus albonotatus to be a continuous breeder, breeding throughout the wet season. Males called for females from the margins of ponds and flooded area, hiding in vegetation or in small depressions such as footprints. Mean clutch size was about 1500 eggs. Females were similar in size to males; females had a mean snout–vent length of and mean body mass of 2.2 g. The same measurements were and 2.4 g for males. Ovaries made about 27% of female bodymass; fecundity increased with the female body size.