Penally station was closed on 15 June 1964, then reopened temporarily during the summers of 1970 and 1971 before being permanently open from 28 February 1972.
Trains call here every two hours in each direction, westwards to and eastwards to, and . There are a pair of through trains to Cardiff each weekday, though late evening services terminate at Carmarthen. There are four trains each way on Sundays. The Summer Saturday GWR service also calls here once a day in both directions.