P35 holin family

The PRD1 Phage P35 Holin Family is a member of Holin Superfamily III. The prototype for this family is the lipid-containing PRD1 enterobacterial phage holin protein P35 encoded by gene XXXV. It is a component of a typical holin-endolysin system which functions to lyse the host bacterial cell.

Structure

P35 holin has 3 transmembrane segments with 5 positively charged residues between TMSs 1 and 2. It has 4 positively charged residues at the C-terminus. It is therefore thought that the N-terminus is in the periplasm and the C-terminus is in the cytoplasm. Homologues of 109 amino acyl residues, which also have 3 putative TMSs, are encoded in the genomes of Xylella fastidiosa strains.

Function

The reaction catalyzed by P35 holin is: