Optimization of Sperm Culture Conditions for Human Assisted Reproductive Technologies
Optimization of Sperm Culture Conditions for Human Assisted Reproductive Technologies is a scholarly work, published in 2018 in ''Global journal of reproductive medicine''. The main subjects of the publication include polycystic ovary syndrome, reproductive technology, male infertility, andrology, fertility preservation, biology, and sperm. Optimizing sperm performance can be a differentiating factor in clinical pregnancy rates for assisted reproductive technology (ART) laboratories.Minimizing cellular stress during routine sperm processing by maintaining a precise and stable environment is of great importance in the field.In commercial media, pH of the external cellular culture environment (pHe) is typically maintained at 7.2-7.4by various buffering reagents, while the internal pH of the cell (pHi) depends on available lactate and amino acids.Combining buffers in solution has been previously shown to be valuable for stabilizing internal and external pH for various biological systems.We hypothesized that a dual-buffer culture medium might improve sperm performance for ART.Here authors demonstrate superior performance of a commercially available dual-buffer solution of HEPES and MOPS: Multipurpose Handling Medium (MHM, Irvine Scientific).Significantly better performance, assessed at 8 and 48 hours, and measured as: sperm viability, total motility, and rapid forward progression, was observed for MHM over single buffer controls.