| Chapter | Charter date and range | Institution | Location | Status | |
| Alpha | 1894–1934 | State University of New York School of Medicine & Biological Medical Science, Buffalo | Buffalo, New York | Withdrew, Merged | |
| Beta First | 1896–1899 | Niagara University | Lewiston, New York | Consolidated | |
| Gamma | 1897–1897; 1903–19xx ? | Union University | Albany, New York | Inactive | |
| Delta | 1898 | University of Colorado Denver | Denver, Colorado | | |
| Epsilon | 1899–19xx ? | University and Bellevue Hospital Medical College | New York City, New York | Inactive | |
| Zeta | 1899–1903 | University of Toronto | Toronto, Ontario, Canada | Inactive | |
| Eta | 1900–1918 | University of Colorado Boulder | Boulder, Colorado | Inactive | |
| Beta Second | 1900–19xx ? | University of Cincinnati | Cincinnati, Ohio | Inactive | |
| Theta | 1901–19xx ? | Cornell University | New York City, New York | Inactive | |
| Iota | 1901–19xx ? | Stanford University | Stanford, California | Inactive | |
| Theta Deuteron | 1902–1910 | Cornell University Medical School | Ithaca, New York | Inactive | |
| Kappa | 1902–1913 | Columbia University | New York City, New York | Inactive | |
| Lambda First | 1903–1906 | Miami Medical College | Cincinnati, Ohio | Inactive | |
| Mu | 1904–1912 | Northwestern University | Evanston, Illinois | Inactive | |
| Nu | 1905–1934 | Medical College of Virginia | Richmond, Virginia | Merged | |
| Xi | 1905–19xx ? | University College of Medicine | Richmond, Virginia | Consolidated | |
| Omicron | 1907–1913 | University of [North Carolina at Chapel Hill] | Chapel Hill, North Carolina | Inactive | |
| Pi | 1908–1934 | University of Pennsylvania | Philadelphia, Pennsylvania | Merged | |
| Rho | 1908–1918 | Jefferson Medical College | Philadelphia, Pennsylvania | Consolidated | |
| Sigma | 1908–1912 | University of Minnesota | Minneapolis, Minnesota | Inactive | |
| Tau | 1908 | North Carolina Medical College | | Consolidated | |
| Upsilon First | 1909–1918 | Medico-Chirurgical College of Philadelphia | Philadelphia, Pennsylvania | Consolidated | |
| Phi | 1911–1916 | Vanderbilt University | Nashville, Tennessee | Inactive | |
| Chi | 1912–1917 | Fordham University | New York City, New York | Inactive | |
| Psi | 1913–19xx ? | University of Maryland | College Park, Maryland | Inactive | |
| Omega | 1914–1934 | University of California | Berkeley, California | Merged | |
| Upsilon Second | 1918–1934 | Temple University School of Medicine | Philadelphia, Pennsylvania | Merged | |
| Lambda Second | 1921–1934 | Georgetown University | Washington, D.C. | Merged | |
| Alpha Alpha | 1922–1934 | Saint Louis University | St. Louis, Missouri | Merged | |
| 19xx– >1928 ? | Ohio State University | Columbus, Ohio | Inactive | |