| State | Name | Hometown | Age1 | Talent | Placement | Awards | Notes |
| Alabama | Scarlotte Deupree | Birmingham | 22 | Vocal | 1st runner-up | Quality of Life 1st runner-up | |
| Alaska | Peggy Willman | Anchorage | 23 | Alaskan Native Dance | | | |
| Arizona | Laura Lawless | Tempe | 23 | Classical Piano | Top 15 | | New York's Junior Miss 1996 |
| Arkansas | Lauren Davidson | El Dorado | 20 | Vocal | | | |
| California | Jennifer Glover | Castro Valley | 23 | Contemporary Vocal | | | Miss California USA 2001 |
| Colorado | Morgan O'Murray | Colorado Springs | 21 | Jazz Dance | | | Miss Colorado Teen USA 1999 |
| Connecticut | Tanisha Brito | New London | 22 | Tap Dance | Top 10 | | Miss Georgia USA 2005 |
| Delaware | Shoha Parekh | Newark | 24 | Bharatnatyam Style Dance | | | |
| District of Columbia | Sarah-Elizabeth Langford | Atlanta, GA | 23 | Gymnastics | | | Miss District of Columbia USA 2005 |
| Florida | Katherine Carson | Sarasota | 20 | Vocal | | | |
| Georgia | Amy Mulkey | Gainesville | 23 | Classical Piano | | | |
| Hawaii | Kehaulani Christian | Pearl City | 23 | Tahitian Dance | | | |
| Idaho | Misty Taylor | Boise | 20 | Classical Vocal | | | |
| Illinois | Erika Harold | Urbana | 22 | Operatic Aria | Winner | Preliminary Interview | |
| Indiana | Tangra Riggle | West Lafayette | 21 | Contemporary Vocal | Top 15 | Preliminary Talent | |
| Iowa | Stephanie Moore | Okoboji | 22 | Vocal | | | |
| Kansas | Jeanne Anne Schroeder | Hutchinson | 24 | Classical Vocal | | | |
| Kentucky | Mary Catherine Correll | Somerset | 21 | Classical Vocal | | | |
| Louisiana | Casey Jo Crowder | Shreveport | 23 | Vocal | | | |
| Maine | Rachel Wadsworth | Hiram | 20 | Rhythm Tap Dance | | | |
| Maryland | Camille Lewis | Silver Spring | 21 | Crossover Classical Violin | 4th runner-up | Overall Talent, Preliminary Talent, Preliminary Evening Gown | |
| Massachusetts | Melanie Correia | Acushnet | 22 | Vocal | Top 10 | | |
| Michigan | Erin Moss | Stevensville | 21 | Flute | | | |
| Minnesota | Allyson Kearns | Eden Prairie | 22 | Self-Choreographed Dance | | | |
| Mississippi | Jennifer Adcock | Hattiesburg | 22 | Piano | Top 10 | Preliminary Swimsuit | Miss Mississippi USA 2005 and Top 10 at Miss USA 2005 |
| Missouri | Shandi Finnessey | Florissant | 24 | Piano Solo | | Preliminary Evening Gown | Miss Missouri USA 2004 and Miss USA 2004 |
| Montana | Heather Rathbun | Darby | 24 | Tae Kwon Do Demonstration | | | |
| Nebraska | Krista Knicely | Omaha | 23 | Jazz Dance | | | |
| Nevada | Teresa Benitez | Reno | 24 | Monologue | 3rd runner-up | Quality of Life, Overall Interview, Preliminary Interview | |
| New Hampshire | Mary Morin | Bedford | 24 | Classical Piano | | | |
| New Jersey | Alicia Luciano | Stanhope | 19 | Lyrical Dance | | Overall Gown, Preliminary Evening Gown | |
| New Mexico | Erin Griggs | Alamogordo | 22 | Violin Solo | | | |
| New York | Tiffany Walker | Brooklyn | 22 | Interpretive Modern Dance | Top 10 | Preliminary Swimsuit | |
| North Carolina | Misty Clymer | Raleigh | 21 | Semi-classical Vocal | Top 15 | | |
| North Dakota | Stacey Thomas | Bismarck | 19 | Jazz en Pointe | | | Miss North Dakota Teen USA 2001 |
| Ohio | Tiffany Haas | Cincinnati | 20 | Classical Vocal | | Quality of Life 2nd runner-up | |
| Oklahoma | Casey Preslar | Tulsa | 22 | Jazz Vocal | 2nd runner-up | Preliminary Talent | |
| Oregon | Brita Stream | Eugene | 22 | Vocal | | | |
| Pennsylvania | Autumn Marisa | Waynesburg | 22 | Lyrical Dance | | | |
| Rhode Island | Gianine Teti | Providence | 22 | Classical Vocal | | | |
| South Carolina | Kelly McCorkle | Greenville | 23 | Lyrical Ballet | Top 15 | | The Amazing Race 7 contestant |
| South Dakota | Vanessa Short Bull | Rapid City | 23 | Ballet en Pointe | | | Miss South Dakota USA 2000 |
| Tennessee | Valli Kugler | Rutherford | 22 | Vocal | | | |
| Texas | Mary Lisa Dalzell | Magnolia | 23 | Flute solo | Top 10 | Preliminary Interview | |
| Utah | Natalie Johnson | Bountiful | 22 | Classical Piano | | | |
| Vermont | Sarah Jo Willey | Bakersfield | 24 | Vocal | | | |
| Virginia | Jennifer Pitts | Arlington | 24 | Contemporary Ballet En Pointe | | | Miss Virginia USA 2005 |
| Washington | Amanda Beers | Richland | 20 | Classical Piano | Top 15 | | |
| West Virginia | Janna Kerns | Terra Alta | 23 | Vocal | | | |
| Wisconsin | Jayme Dawicki | New Berlin | 22 | Classical Piano | | | |
| Wyoming | Beth Holland | Gillette | 23 | Vocal | | | |
