Middlebrook 7H9 Broth


Middlebrook 7H9 broth is a liquid growth medium specially used for culture of Mycobacterium species, notably Mycobacterium tuberculosis.

Composition

Middlebrook 7H9 broth supports the growth of mycobacterial species when supplemented with nutrients such as glycerol, oleic acid, albumin, and dextrose, except for Mycobacterium bovis, which is inhibited by glycerol. Cultures should be read within 5–7 days after inoculation and once a week thereafter for up to 8 weeks.
Middlebrook broth is commonly used in the preparation of inocula for antimicrobial assays, biochemical tests, and maintenance of stock strains.
Additionally, 7H9 broth is used as a medium in the mycobacteria growth indicator tube.