Measuring Protein Mobility by Photobleaching GFP Chimeras in Living Cells
Measuring Protein Mobility by Photobleaching GFP Chimeras in Living Cells is a scholarly work by Jennifer Lippincott-Schwartz and Erik Lee Snapp, published in 2003 in ''Current protocols in cell biology''. The main subjects of the publication include chemistry, fluorescence recovery after photobleaching, green fluorescent protein, Förster resonance energy transfer, fluorescence microscope, biophysics, protein expression (biotechnology), fluorescence, confocal microscopy, Bioimage informatics, photobleaching, and chimera. AbstractThis unit describes fluorescence recovery after photobleaching (FRAP) and fluorescence loss in photobleaching (FLIP) using commercially available confocal scanning laser microscopy (CSLM).