MRS agar

De Man–'Rogosa–Sharpe agar, often abbreviated to MRS', is a selective culture medium designed to favour the luxuriant growth of Lactobacilli for lab study. Developed in 1960, this medium was named for its inventors,,, and. It contains sodium acetate, which suppresses the growth of many competing bacteria. This medium has a clear brown colour.

Typical composition

MRS agar typically contains :

1.0% peptone
1.0% beef extract
0.4% yeast extract
2.0% glucose
0.5% sodium acetate trihydrate
0.1% polysorbate 80
0.2% dipotassium hydrogen phosphate
0.2% triammonium citrate
0.02% magnesium sulfate heptahydrate
0.005% manganese sulfate tetrahydrate
1.0% agar
pH adjusted to 6.2 at 25 °C

The yeast/meat extracts and peptone provide sources of carbon, nitrogen, and vitamins for general bacterial growth. The yeast extract also contains vitamins and amino acids required by Lactobacilli. Polysorbate 80 is a surfactant which assists in nutrient uptake by Lactobacilli. Magnesium sulfate and manganese sulfate provide cations used in metabolism.