MLS (disambiguation)
- Head gasket
- Leibniz Society of Sciences
- MLS (Making Life Simple) S.A.
- MSL (disambiguation)
- Major League Soccer
- Maritime Launch Services
- Martin-Luther-Schule
- Master of Arts in Liberal Studies
- Master of Library and Information Science
- Master of Studies in Law
- Maximum length sequence
- Medical laboratory scientist
- Messaging Layer Security
- Michigan Lutheran Seminary
- Microwave landing system
- Microwave limb sounder
- Mozilla Location Service
- Multilayer switch
- Multilevel security
- Multiple listing service