| Name | Image | Town/City | Region | Type | Summary |
| Basildon Park | | Basildon | West Berkshire | Historic house | Operated by the National Trust, 18th century Georgian mansion, gardens, park |
| Berkshire Medical Heritage Centre | | Reading | Reading | Medical | In the 1881 built laundry building of the Royal Berkshire Hospital |
| Cole Museum of Zoology | | Reading | Reading | Natural history | Part of the University of Reading, complete tour of the diversity of the animal kingdom |
| Eton College Natural History Museum | | Eton | Windsor and Maidenhead | Natural history | Part of Eton College, mounted animals, fossils, fish |
| Frogmore House | | Windsor | Windsor and Maidenhead | Historic house | Royal country residence |
| Look Out Discovery Centre | | Bracknell | Bracknell Forest | Science | hands-on science and nature exhibits |
| Maidenhead Heritage Centre | | Maidenhead | Windsor and Maidenhead | Local | Local history, culture |
| Museum of Berkshire Aviation | | Woodley | Wokingham | Aviation | Locally-made aircraft |
| Museum of English Rural Life | | Reading | Reading | Agriculture | Part of the University of Reading, rural history, crafts, farming |
| Museum of Eton Life | | Eton | Windsor and Maidenhead | Education | Part of Eton College, school history, traditions and graduates |
| Museum of Reading | | Reading | Reading | Multiple | Local history, culture, art, Roman artifacts |
| Riverside Museum at Blake's Lock | | Reading | Reading | Multiple | Turbine machinery, gypsy life, art, mill wheel |
| Shaw House | | Shaw | West Berkshire | Historic house | Elizabethan grand house with five unfurnished rooms, exhibits about the house |
| Slough Museum | | Slough | Slough | Local | Local history, culture |
| South Hill Park Arts Centre | | Bracknell | Bracknell Forest | Art | |
| Stanley Spencer Gallery | | Cookham | Windsor and Maidenhead | Art | Works by English painter Sir Stanley Spencer |
| Thames Valley Police Museum | | Sulhamstead | West Berkshire | Law enforcement | Open by appointment |
| Ure Museum of Greek Archaeology | | Reading | Reading | Archaeology | Part of the University of Reading, Greek and Etruscan ceramics and terracottas, Egyptian artifacts |
| West Berkshire Museum | | Newbury | West Berkshire | Local | Local history, culture |
| Windsor and Royal Borough Museum | | Windsor | Windsor and Maidenhead | Local | Local history, culture, formerly the Royal Borough Museum Collection |
| Windsor Castle | | Windsor | Windsor and Maidenhead | Historic house | One of the official residences of the British monarch, includes significant art collection |