| Name | Mark | System | From | To | Successor | Notes |
| Abbeville–Grimes Railway | AG | | 1989 | 1994 | A&G Railroad | - |
| Abbeville Southern Railway | | ACL | 1892 | 1901 | Savannah, Florida and Western Railway | - |
| A&G Railroad | AG | | 1994 | 1996 | Bay Line Railroad | - |
| Alabama Central Railroad (1906) | | | 1906 | 1961 | N/A | Commemorative website: http://www.alabamacentralrailroad.com/home/) |
| Alabama Central Railroad | | SOU | 1871 | 1881 | East Tennessee, Virginia and Georgia Railroad | - |
| Alabama Central Railway | | | 1903 | 1939 | N/A | - |
| Alabama and Chattanooga Railroad | | SOU | 1868 | 1877 | Alabama Great Southern Railroad | - |
| Alabama and Florida Railroad | | L&N | 1850 | 1868 | Pensacola and Louisville Railroad | - |
| Alabama and Florida Railroad | | L&N | 1898 | 1900 | Louisville and Nashville Railroad | - |
| Alabama and Florida Railroad | | | 1936 | 1941 | N/A | - |
| Alabama and Florida Railroad | AFLR | | 1986 | 1992 | Alabama and Florida Railway | - |
| Alabama and Florida Railway | AF | | 1992 | 2011 | N/A | - |
| Alabama, Florida and Gulf Railroad | | | 1917 | 1936 | Alabama and Florida Railroad | - |
| Alabama, Florida and Southern Railroad | | | 1910 | 1917 | Alabama, Florida and Gulf Railroad | - |
| Alabama Great Southern Railroad | AGS | SOU | 1877 | | | Still exists as an operating subsidiary of the Norfolk Southern Railway |
| Alabama Industrial Railroad | AIRI | | 1984 | 1985 | Huntsville and Madison County Railroad Authority | - |
| Alabama Midland Railway | | ACL | 1887 | 1901 | Savannah, Florida and Western Railway | - |
| Alabama Mineral Railroad | | L&N | 1890 | 1903 | Louisville and Nashville Railroad | - |
| Alabama and Mississippi Railroad | A&M | | 1902 | 1922 | Mississippi and Alabama Railroad | - |
| Alabama and Mississippi Rivers Railroad | | SOU | 1850 | 1864 | Selma and Meridian Railroad | - |
| Alabama Northern Railway | ABN | | 1904 | 1925 | | - |
| Alabama and North Western Railroad | | | 1912 | 1934 | N/A | - |
| Alabama Railroad | ALAB | | 1991 | 2022 | N/A | |
| Alabama Southern Railroad | | | 1985 | 1985 | N/A | Never operated |
| Alabama, Tennessee and Northern Railroad | | SLSF | 1918 | 1971 | St. Louis – San Francisco Railway | - |
| Alabama, Tennessee and Northern Railroad | | SLSF | 1906 | 1913 | Alabama, Tennessee and Northern Railway | - |
| Alabama, Tennessee and Northern Railway | | SLSF | 1913 | 1918 | Alabama, Tennessee and Northern Railroad | - |
| Alabama and Tennessee River Railroad | | SOU | 1848 | 1866 | Selma, Rome and Dalton Railroad | - |
| Alabama Terminal and Improvement Company | | ACL | 1889 | 1889 | Alabama Midland Railway | - |
| Alabama Terminal Railroad | | ACL | 1907 | 1916 | Atlanta, Birmingham and Atlantic Railway | - |
| Alabama and Tombigbee Railroad | | | 1898 | | N/A | - |
| Alabama Transit Company | | SOU | 1869 | 1870 | Elyton and Aberdeen Railroad | - |
| Alabama Western Railroad | | IC | 1905 | 1913 | Chicago, St. Louis and New Orleans Railroad | - |
| Americus, Preston and Lumpkin Railroad | | SAL | 1884 | 1888 | Savannah, Americus and Montgomery Railway | - |
| Andalusia and Conecuh Railroad | ACRC | | 1983 | | | Still exists as a lessor of the Three Notch Railroad |
| Andalusia, Florida and Gulf Railway | | | 1919 | 1925 | N/A | - |
| Anniston and Atlantic Railroad | | L&N | 1883 | 1890 | Alabama Mineral Railroad | - |
| Anniston and Cincinnati Railroad | | L&N | 1887 | 1890 | Alabama Mineral Railroad | - |
| Anniston Terminal Railroad | | L&N | 1888 | 1890 | Anniston and Cincinnati Railroad | - |
| Ashland Railway | | | 1925 | 1940 | N/A | - |
| Atlanta and Birmingham Air Line Railway | | SAL | 1903 | 1909 | Seaboard Air Line Railway | - |
| Atlanta, Birmingham and Atlantic Railroad | | ACL | 1905 | 1916 | Atlanta, Birmingham and Atlantic Railway | - |
| Atlanta, Birmingham and Atlantic Railway | | ACL | 1916 | 1927 | Atlanta, Birmingham and Coast Railroad | - |
| Atlanta, Birmingham and Coast Railroad | | ACL | 1927 | 1946 | Atlantic Coast Line Railroad | - |
| Atlanta and St. Andrews Bay Railway | A&SA, ASAB | | 1906 | 1993 | Bay Line Railroad | - |
| Atlantic Coast Line Railroad | ACL | ACL | 1902 | 1967 | Seaboard Coast Line Railroad | - |
| Bay Minette and Fort Morgan Railroad | | L&N | 1904 | 1921 | Louisville and Nashville Railroad | - |
| Belt Line Railway | | ACL | 1889 | 1943 | Atlantic Coast Line Railroad | - |
| Birmingham and Atlanta Air Line Railway | | SAL | 1902 | 1903 | East and West Railroad | - |
| Birmingham and Atlantic Railroad | | | 1890 | 1919 | N/A | - |
| Birmingham Belt Railroad | | SLSF | 1899 | 1968 | St. Louis – San Francisco Railway | - |
| Birmingham Mineral Railroad | | L&N | 1884 | 1904 | Louisville and Nashville Railroad | - |
| Birmingham, Selma and Mobile Railroad | | | 1913 | 1931 | N/A | - |
| Birmingham, Selma and New Orleans Railway | | L&N | 1886 | 1902 | Louisville and Nashville Railroad | - |
| Birmingham, Selma and Pensacola Railroad | | | 1910 | 1913 | Birmingham, Selma and Mobile Railroad | - |
| Birmingham, Sheffield and Tennessee River Railway | | SOU | 1889 | 1895 | Northern Alabama Railway | - |
| Birmingham and Southeastern Railway | B&SE | | 1911 | 1965 | N/A | - |
| Birmingham and Tennessee River Railroad | | SOU | 1884 | 1885 | Sheffield and Birmingham Railroad | - |
| Birmingham Southern Railroad | BS | | 1899 | 2012 | Birmingham Terminal Railway | - |
| Birmingham Terminal Company | BTC | CG/ IC/ SAL/ SLSF/ SOU | | | | - |
| Birmingham and Tuscaloosa Railroad | | L&N | 1915 | 1915 | Louisville and Nashville Railroad | - |
| Brierfield, Blockton and Birmingham Railway | | SOU | 1889 | 1889 | East Tennessee, Virginia and Georgia Railway | - |
| Burlington Northern Inc. | BN | | 1980 | 1981 | Burlington Northern Railroad | - |
| Burlington Northern Railroad | BN | | 1981 | 1996 | Burlington Northern and Santa Fe Railway | - |
| Cahaba, Marion and Greensboro Railroad | | SOU | 1860 | 1868 | Selma, Marion and Memphis Railroad | - |
| Canton, Aberdeen and Nashville Railroad in Alabama | | IC | 1898 | 1913 | Chicago, St. Louis and New Orleans Railroad | - |
| Carrollton Short Line Railway | | SLSF | 1897 | 1906 | Alabama, Tennessee and Northern Railroad | - |
| Central of Georgia Railway | CG | CG | 1895 | 1971 | Central of Georgia Railroad | - |
| Chattahoochee and Gulf Railroad | CHAT | | 2003 | 2006 | Chattahoochee Bay Railroad | - |
| Chattahoochee and Gulf Railroad | | CG | 1899 | 1948 | Central of Georgia Railway | - |
| Chattahoochee Valley Railroad | | | 1895 | 1900 | Chattahoochee Valley Railway | - |
| Chattahoochee Valley Railway | CHV | | 1900 | 1992 | N/A | - |
| Chattanooga Southern Railroad | | SOU | 1896 | 1911 | Tennessee, Alabama and Georgia Railroad | - |
| Chattanooga Southern Railway | | SOU | 1890 | 1895 | Chattanooga Southern Railroad | - |
| Cheney Railroad | CHNY | | 1989 | 1996 | N/A | - |
| Chicago, St. Louis and New Orleans Railroad | | IC | 1913 | 1951 | Illinois Central Railroad | - |
| Cincinnati, Selma and Mobile Railway | | SOU | 1881 | 1890 | East Tennessee, Virginia and Georgia Railway | - |
| Columbus, Fayette and Decatur Railroad | | SOU | 1871 | 1881 | Georgia Pacific Railway | - |
| Columbus and Western Railway | | CG | 1880 | 1888 | Savannah and Western Railroad | - |
| Decatur, Chesapeake and New Orleans Railway | | L&N | 1887 | 1893 | Middle Tennessee and Alabama Railway | - |
| DeKalb and Western Railroad | | | 1916 | | N/A | - |
| East Alabama Railway | | CG | 1880 | 1888 | Savannah and Western Railroad | - |
| East Alabama and Cincinnati Railroad | | CG | 1868 | 1880 | East Alabama and Cincinnati Railway | - |
| East Alabama and Cincinnati Railway | | CG | 1880 | 1880 | East Alabama Railway | - |
| East Tennessee, Virginia and Georgia Railroad | | SOU | 1881 | 1886 | East Tennessee, Virginia and Georgia Railway | - |
| East Tennessee, Virginia and Georgia Railway | | SOU | 1886 | 1894 | Southern Railway | - |
| East and West Railroad | | SAL | 1894 | 1903 | Atlanta and Birmingham Air Line Railway | - |
| East and West Railroad of Alabama | | SAL | 1882 | 1893 | East and West Railroad | - |
| Eastern Railway of Alabama | | ACL | 1901 | 1906 | Atlanta, Birmingham and Atlantic Railroad | - |
| Elyton and Aberdeen Railroad | | SOU | 1870 | 1882 | Georgia Pacific Railway | - |
| Ensley Southern Railway | | SOU | 1900 | 1926 | Southern Railway, Warrior River Terminal Company | - |
| Escambia Railroad | | | | | | - |
| Eufaula and East Alabama Railway | | CG | 1887 | 1888 | Savannah and Western Railroad | - |
| Eufaula and Clayton Railway | | CG | 1883 | 1888 | Savannah and Western Railroad | - |
| Florence Bridge Company | | SOU | 1832 | 1850 | Memphis and Charleston Railroad | - |
| Florence Railroad and Improvement Company | | L&N | 1888 | 1897 | Louisville and Nashville Railroad | - |
| Florida and Alabama Railroad | | | | | | - |
| Florida, Alabama and Gulf Railroad | | | 1911 | 1919 | Andalusia, Florida and Gulf Railway | - |
| Gadsden and Attalla Railroad | | SOU | 1876 | 1904 | Southern Railway | - |
| Gadsden and Birmingham Railroad | | SOU | 1910 | 1911 | Tennessee, Alabama and Georgia Railroad | - |
| Gainesville and Mississippi Railroad | | GM&O | 1852 | 1854 | Mississippi, Gainesville and Tuscaloosa Railroad | - |
| Georgia and Alabama Railroad | GAAB | | 1989 | 1995 | Georgia Southwestern Railroad | - |
| Georgia and Alabama Railway | | SAL | 1895 | 1902 | Seaboard Air Line Railway | - |
| Georgia Pacific Railroad | | SOU | 1876 | 1882 | Georgia Pacific Railway | - |
| Georgia Pacific Railway | | SOU | 1882 | 1894 | Southern Railway | - |
| Georgia Western Railroad | | SOU | 1860 | 1877 | Georgia Pacific Railroad | - |
| Girard Railroad | | CG | 1845 | 1854 | Mobile and Girard Railroad | - |
| Goodwater and Birmingham Railroad | | CG | 1886 | 1887 | Columbus and Western Railway | - |
| Gulf, Florida and Alabama Railway | DWR | SLSF | 1911 | 1922 | Muscle Shoals, Birmingham and Pensacola Railway | - |
| Gulf and Mississippi Railroad | GMSR | | 1985 | 1988 | SouthRail Corporation | - |
| Gulf, Mobile and Northern Railroad | GM&N | GM&O | 1915 | 1940 | Gulf, Mobile and Ohio Railroad | - |
| Gulf, Mobile and Ohio Railroad | GM&O, GMO | GM&O | 1940 | 1972 | Illinois Central Gulf Railroad | - |
| Gulf Ports Terminal Railway | | | 1916 | 1926 | N/A | - |
| Gulf Terminal Company | | GM&O/ SOU | | | | - |
| Hartford and Slocomb Railroad | HS | | 1953 | 1992 | H&S Railroad | - |
| Hayneville and Montgomery Railroad | | | 1905 | 1917 | | - |
| H&S Railroad | HS | | 1992 | 2006 | Chattahoochee Bay Railroad | - |
| Huntsville and Elora Railroad | | L&N | 1887 | 1887 | Nashville, Chattanooga and St. Louis Railway | - |
| Illinois Central Railroad | IC | IC | 1899 | 1972 | Illinois Central Gulf Railroad | - |
| Illinois Central Gulf Railroad | ICG | | 1972 | 1988 | Illinois Central Railroad | - |
| Indiana, Alabama and Texas Railroad | | L&N | 1881 | 1887 | Louisville and Nashville Railroad | - |
| Inland Waterways Corporation | | | 1948 | 1966 | Birmingham Southern Railroad | - |
| Jefferson Warrior Railroad | | | 1985 | 2009 | Alabama Warrior Railway | - |
| Kansas City, Memphis and Birmingham Railroad | | SLSF | 1887 | 1928 | St. Louis – San Francisco Railway | - |
| Kansas City Southern Railway | KCS | | 1994 | 2005 | Alabama Southern Railroad | - |
| Long Branch Coal Railroad | | L&N | 1901 | 1957 | Louisville and Nashville Railroad | - |
| Louisville and Nashville Railroad | L&N, LN | L&N | 1871 | 1983 | Seaboard System Railroad | - |
| Manistee and Repton Railroad | | L&N | 1910 | 1965 | Louisville and Nashville Railroad | - |
| Marion Railroad | | SOU | 1854 | 1858 | Marion and Cahaba Railroad | - |
| Marion and Alabama River Transportation Company | | SOU | 1850 | 1854 | Marion Railroad | - |
| Marion and Cahaba Railroad | | SOU | 1858 | 1860 | Cahaba, Marion and Greensboro Railroad | - |
| Mary Lee Railroad | | | | 1985 | Jefferson Warrior Railroad | private |
| Meridian and Bigbee Railroad | MBRR, MB | | 1952 | 1997 | M&B Railroad | - |
| Meridian and Bigbee River Railway | | | 1917 | 1952 | Meridian and Bigbee Railroad | - |
| Memphis and Birmingham Railroad | | SLSF | 1886 | 1887 | Kansas City, Memphis and Birmingham Railroad | - |
| Memphis and Charleston Railroad | | SOU | 1850 | 1898 | Southern Railway | - |
| Middle Tennessee and Alabama Railway | | L&N | 1893 | 1897 | Nashville, Chattanooga and St. Louis Railway | - |
| Mississippi and Alabama Railroad | | | 1922 | 1950 | N/A | - |
| Mississippi Central Railroad | MSC | IC | 1921 | 1924 | N/A | Trackage rights over the Gulf, Mobile and Northern Railroad to Mobile |
| Mississippi, Gainesville and Tuscaloosa Railroad | | GM&O | 1854 | 1870 | Mobile and Ohio Railroad | - |
| Mobile and Alabama Grand Trunk Railroad | | SOU | 1866 | 1886 | Mobile and West Alabama Railroad | - |
| Mobile and Bay Shore Railway | | GM&O | 1898 | 1903 | Mobile and Ohio Railroad | - |
| Mobile and Birmingham Railroad | | SOU | 1895 | | | Still exists as a nonoperating subsidiary of the Norfolk Southern Railway |
| Mobile and Birmingham Railway | | SOU | 1887 | 1895 | Mobile and Birmingham Railroad | - |
| Mobile and Girard Railroad | | CG | 1854 | 1895 | Central of Georgia Railway | - |
| Mobile and Great Northern Railroad | | L&N | 1856 | 1868 | Mobile and Montgomery Railroad | - |
| Mobile and Gulf Railroad | MG | | 1925 | 1984 | N/A | - |
| Mobile, Hattiesburg and Jackson Railroad | | GM&O | 1887 | 1888 | Mobile, Jackson and Kansas City Railroad | - |
| Mobile, Jackson and Kansas City Railroad | | GM&O | 1888 | 1909 | New Orleans, Mobile and Chicago Railroad | - |
| Mobile and Montgomery Railroad | | L&N | 1868 | 1874 | Mobile and Montgomery Railway | - |
| Mobile and Montgomery Railway | | L&N | 1874 | 1900 | Louisville and Nashville Railroad | - |
| Mobile and Northwestern Railroad | | GM&O | 1870 | 1891 | Mobile, Jackson and Kansas City Railroad | - |
| Mobile and Ohio Railroad | | GM&O | 1848 | 1940 | Gulf, Mobile and Ohio Railroad | - |
| Mobile Terminal and Railway Company | | SLSF | 1910 | 1913 | Alabama, Tennessee and Northern Railway | - |
| Mobile and West Alabama Railroad | | SOU | 1885 | 1887 | Mobile and Birmingham Railway | - |
| Mobile and Western Railroad | | GM&O | 1906 | 1907 | Mobile and Ohio Railroad | - |
| Montgomery Railroad | | ACL/ L&N | 1834 | 1842 | Montgomery and West Point Railroad | - |
| Montgomery and Eufaula Railroad | | CG | 1860 | 1879 | Montgomery and Eufaula Railway | - |
| Montgomery and Eufaula Railway | | CG | 1879 | 1895 | Central of Georgia Railway | - |
| Montgomery and Florida Railway | | ACL | 1886 | 1888 | Northwestern and Florida Railroad | - |
| Montgomery, Hayneville and Camden Railroad | | L&N | 1890 | 1900 | Louisville and Nashville Railroad | - |
| Montgomery and Prattville Railroad | | L&N | 1895 | 1896 | Louisville and Nashville Railroad | - |
| Montgomery Southern Railway | | ACL | 1880 | 1886 | Montgomery and Florida Railway | - |
| Montgomery and West Point Railroad | | ACL/ L&N | 1843 | 1870 | Western Railroad of Alabama | - |
| Muscle Shoals, Birmingham and Pensacola Railroad | MSBP | SLSF | 1924 | 1928 | St. Louis – San Francisco Railway | - |
| Muscle Shoals, Birmingham and Pensacola Railway | MSBP | SLSF | 1922 | 1924 | Muscle Shoals, Birmingham and Pensacola Railroad | - |
| Nashville and Chattanooga Railroad | | L&N | 1850 | 1873 | Nashville, Chattanooga and St. Louis Railway | - |
| Nashville, Chattanooga and St. Louis Railway | N&C, NC | L&N | 1873 | 1957 | Louisville and Nashville Railroad | - |
| Nashville and Decatur Railroad | | L&N | 1866 | 1990 | CSX Transportation | - |
| Nashville, Florence and Sheffield Railway | | L&N | 1887 | 1900 | Louisville and Nashville Railroad | - |
| New and Old Decatur Belt and Terminal Railroad | | L&N | 1890 | 1892 | Louisville and Nashville Railroad | - |
| New Orleans, Mobile and Chattanooga Railroad | | L&N | 1866 | 1871 | New Orleans, Mobile and Texas Railroad | - |
| New Orleans, Mobile and Chicago Railroad | | GM&O | 1909 | 1915 | Gulf, Mobile and Northern Railroad | - |
| New Orleans, Mobile and Texas Railroad | | L&N | 1871 | 1881 | Louisville and Nashville Railroad | - |
| New Orleans and Selma Railroad | | L&N | 1866 | 1868 | New Orleans and Selma Railroad and Immigration Association | - |
| New Orleans and Selma Railroad and Immigration Association | | L&N | 1868 | 1886 | Birmingham, Selma and New Orleans Railway | - |
| North Alabama Railroad | | L&N | 1900 | 1910 | Louisville and Nashville Railroad | - |
| North East and South West Alabama Railroad | | SOU | 1853 | 1868 | Alabama and Chattanooga Railroad | - |
| Northern Alabama Railway | | SOU | 1895 | 1939 | Southern Railway | - |
| Northwestern Railroad of Alabama | | SOU | 1854 | 1868 | Selma, Marion and Memphis Railroad | - |
| Northwestern and Florida Railroad | | ACL | 1888 | 1889 | Alabama Terminal and Improvement Company | - |
| Oak Grove and Georgetown Railroad | | | 1904 | 1927 | N/A | - |
| Oneonta and Attalla Railroad | | L&N | 1900 | 1905 | Louisville and Nashville Railroad | - |
| Opelika and Talladega Railroad | | CG | 1854 | 1861 | Opelika and Tuscumbia Railroad | - |
| Opelika and Tuscumbia Railroad | | CG | 1861 | 1866 | Savannah and Memphis Railroad | - |
| Pensacola, Mobile and New Orleans Railway | | | 1907 | 1917 | Gulf Ports Terminal Railway | - |
| Pensacola and Selma Railroad | | L&N | 1880 | 1880 | Louisville and Nashville Railroad | - |
| Pine Belt Southern Railroad | PBRR | | 1995 | 2001 | N/A | - |
| Plant System | | ACL | 1894 | 1902 | Atlantic Coast Line Railroad | - |
| Richmond and Danville Railroad | | SOU | 1889 | 1894 | Southern Railway | - |
| Rome and Decatur Railroad | | SOU | 1882 | 1889 | East Tennessee, Virginia and Georgia Railway | - |
| St. Louis and San Francisco Railroad | | SLSF | 1903 | 1916 | St. Louis – San Francisco Railway | - |
| St. Louis – San Francisco Railway | SLSF | SLSF | 1916 | 1980 | Burlington Northern Inc. | - |
| Savannah, Americus and Montgomery Railway | | SAL | 1888 | 1895 | Georgia and Alabama Railway | - |
| Savannah, Florida and Western Railway | | ACL | 1901 | 1902 | Atlantic Coast Line Railroad | - |
| Savannah and Memphis Railroad | | CG | 1866 | 1880 | Columbus and Western Railway | - |
| Savannah and Western Railroad | | CG | 1885 | 1895 | Central of Georgia Railway | - |
| Seaboard Railroad of Alabama | | | 1890 | 1900 | Tombigbee and Northern Railway | - |
| Seaboard Air Line Railroad | SAL | SAL | 1946 | 1967 | Seaboard Coast Line Railroad | - |
| Seaboard Air Line Railway | | SAL | 1900 | 1945 | Seaboard Air Line Railroad | - |
| Seaboard Coast Line Railroad | SCL | | 1967 | 1983 | Seaboard System Railroad | - |
| Seaboard System Railroad | SBD | | 1983 | 1986 | CSX Transportation | - |
| Selma and Gulf Railroad | | L&N | 1858 | 1879 | Pensacola and Selma Railroad | - |
| Selma and Greensboro Railroad | | SOU | 1878 | 1881 | Cincinnati, Selma and Mobile Railway | - |
| Selma, Marion and Memphis Railroad | | SOU | 1868 | 1878 | Selma and Greensboro Railroad | - |
| Selma and Meridian Railroad | | SOU | 1864 | 1871 | Alabama Central Railroad | - |
| Selma, Rome and Dalton Railroad | | SOU | 1866 | 1880 | East Tennessee, Virginia and Georgia Railroad | - |
| Sheffield and Birmingham Railroad | | SOU | 1885 | 1887 | Sheffield and Birmingham Coal, Iron and Railway Company | - |
| Sheffield and Birmingham Coal, Iron and Railway Company | | SOU | 1887 | 1889 | Birmingham, Sheffield and Tennessee River Railway | - |
| Sheffield and Tuscumbia Railroad | | L&N | 1895 | 1896 | Louisville and Nashville Railroad | - |
| Sheffield and Tuscumbia Street Railway | | L&N | 1886 | 1895 | Sheffield and Tuscumbia Railroad | - |
| South and North Alabama Railroad | | L&N | 1854 | 1914 | Louisville and Nashville Railroad | - |
| Southern Railway | SOU | SOU | 1894 | 1990 | Norfolk Southern Railway | - |
| Southern Alabama Railroad | SUAB | | 1988 | 2001 | Conecuh Valley Railroad | - |
| Southern Alabama Railroad | | L&N | 1899 | 1899 | Louisville and Nashville Railroad | - |
| SouthRail Corporation | SR | | 1988 | 1994 | Kansas City Southern Railway | - |
| Southwestern Railroad | | CG | 1859 | | | Owned by Central of Georgia Railway; still exists as a lessor of Norfolk Southern Railway operating subsidiary Central of Georgia Railroad |
| Southwestern Alabama Railway | | ACL | 1896 | 1901 | Savannah, Florida and Western Railway | - |
| Sumter and Choctaw Railway | SC | | 1904 | 1983 | Alabama Southern Railroad | - |
| Sylacauga and Wetumpka Railroad | | | 1902 | 1911 | | - |
| Talladega and Coosa Valley Railroad | | | 1883 | 1890 | Birmingham and Atlantic Railroad | - |
| Tallassee and Montgomery Railway | | | 1895 | 1912 | Birmingham and Southeastern Railway | - |
| Tennessee and Alabama Railroad | | L&N | 1887 | 1887 | Nashville, Florence and Sheffield Railway | - |
| Tennessee and Alabama Central Railroad | | L&N | 1853 | 1866 | Nashville and Decatur Railroad | - |
| Tennessee, Alabama and Georgia Railroad | | SOU | 1911 | 1922 | Tennessee, Alabama and Georgia Railway | - |
| Tennessee, Alabama and Georgia Railway | TA&G, TAG | SOU | 1922 | | | Still exists as an operating subsidiary of the Norfolk Southern Railway |
| Tennessee Coal and Iron Railroad | | | | | | - |
| Tennessee and Coosa Railroad | | L&N | 1844 | 1891 | Nashville, Chattanooga and St. Louis Railway | - |
| Tennessee Valley Railroad | | SOU | 1848 | 1851 | Memphis and Charleston Railroad | - |
| Tombigbee and Northern Railway | | | 1900 | 1904 | Tombigbee Valley Railroad | - |
| Tombigbee Valley Railroad | | SLSF | 1904 | 1913 | Alabama, Tennessee and Northern Railway | - |
| Tredegar Mineral Railway | | SAL | 1891 | 1900 | East and West Railroad | - |
| Tuscaloosa Mineral Railroad | | L&N | 1910 | 1915 | Birmingham and Tuscaloosa Railroad | - |
| Tuscumbia Railway | | SOU | 1830 | 1850 | Memphis and Charleston Railroad | - |
| Tuscumbia, Courtland and Decatur Railroad | | SOU | 1832 | 1847 | Tennessee Valley Railroad | - |
| Tuskegee Railroad | TK | | 1860 | 1963 | N/A | - |
| Tyson Railroad | TSNR | | 1989 | 1992 | CSX Transportation | - |
| Union Springs and Northern Railway | | | 1901 | 1911 | Birmingham and Southeastern Railway | - |
| Vicksburg and Brunswick Railroad | | CG | 1867 | 1879 | Eufaula and Clayton Railway | - |
| Vredenburgh Saw Mill Company | | | | | | - |
| Warrior River Terminal Company | | | 1926 | 1948 | Inland Waterways Corporation | - |
| Warrior Southern Railway | | | 1901 | 1938 | Twin Seam Mining Company | Owned by Mobile and Ohio Railroad |
| Washington and Choctaw Railway | | | 1910 | 1927 | N/A | - |
| Western Railroad of Alabama | | ACL/ L&N | 1860 | 1883 | Western Railway of Alabama | - |
| Western Railway of Alabama | WA | ACL/ L&N | 1883 | 2002 | CSX Transportation | - |
| Wills Valley Railroad | | SOU | 1852 | 1868 | Alabama and Chattanooga Railroad | - |
| Winchester and Alabama Railroad | | SOU | 1850 | 1875 | Memphis and Charleston Railroad | - |
| Woodstock and Blocton Railway | | L&N/ SOU | 1906 | | | Still exists; owned by CSX Transportation and the Norfolk Southern Railway but operated by the latter |
| Woodward Iron Company | | | | | | private |
| Yellow River Railroad | | L&N | 1887 | 1906 | Louisville and Nashville Railroad | - |