John Harley

• John Harley (bishop, died 1558), earlier bishop of Hereford
• John Harley (bishop, died 1788), dean of Windsor and bishop of Hereford
• John Pritt Harley, English comic actor
• John Harley (physician), English physician, geologist, and botanist
• John Harley (footballer), Scottish-born Uruguayan footballer
• John Brian Harley, geographer, cartographer, and map historian
• John B. Harley, president of Fordham University, 1843–1845
• Jack Harley, British biologist