Tardiff joined the faculty at her alma mater, Albert Einstein College of Medicine, in 2001 as an assistant professor of medicine and physiology and biophysics. During her tenure at the institution, she studied the basic molecular mechanisms that underlie the pathogenesis of cardiomyopathies caused by mutations in the cardiac thin filament in her lab. She stayed there until 2012 when she accepted an endowed chair position at the University of Arizona College of Medicine – Tucson. In the role of the Steven M. Gootter Endowed Chair for the Prevention of Sudden Cardiac Death, Tardiff planned on establishing a clinic to focus on hypertrophic cardiomyopathy patients. She also launched several initiatives in the department of medicine including grant review and incentive programs for junior faculty members and developing the first CDA Research Symposium. In recognition of her efforts, she received a 2017 Faculty Mentoring Award along with Jarrod M. Mosier and C. Kent Kwoh. Tardiff was named to the scientific advisory board of the Marfan Foundation.