James Knowles
James Knowles may refer to:
- James Sheridan Knowles, Irish dramatist and actor
- James [Thomas Knowles (1806–1884)], English architect
- James Thomas Knowles (1831–1908), English architect and editor of The Nineteenth Century
- James Knowles (aviator), World War I ace
- James Knowles (footballer, born 1881), English footballer
- James Knowles (lexicographer), Irish schoolteacher and lexicographer, father of James Sheridan Knowles
- Jim Knowles (American football), defensive coordinator at Penn State University
- Jim Knowles (football manager), manager of Tranmere Rovers, 1936–1939
- Jimmy Knowles (baseball), baseball player
- James Knowles (murderer), Ku Klux Klan member responsible for the lynching of Michael Donald
- James Knowles III, mayor of Ferguson, Missouri
- James Hinton Knowles, British missionary to Kashmir
- Jimmy Knowles (footballer), English footballer playing for Accrington Stanley F.C.