James Johnstone
James Johnstone may refer to:
- James Johnstone, 1st Earl of Hartfell, Scottish peer and royalist
- James Johnstone, 2nd Marquess of Annandale, Scottish peer
- Sir James Johnstone, 3rd Baronet, Scottish MP for Dumfries Burghs 1743–1754
- Sir James Johnstone, 4th Baronet, Scottish MP for Dumfries Burghs 1784–1790 and Weymouth & Melcombe Regis
- James Johnstone , master, later lieutenant, aboard HMS Chatham during George Vancouver's 1791–95 expedition
- James Hope-Johnstone, 3rd Earl of Hopetoun, Scottish representative peer and Lord Lieutenant of Linlithgowshire
- Chevalier de Johnstone, actually James Johnstone, Jacobite army officer
- James Johnstone, Scottish MP for Clackmannanshire & Kinross-shire
- James Johnstone , British newspaper proprietor
- James Johnstone , British biologist and oceanographer
- James Johnstone , New Zealand businessman and stock breeder
- Jim Johnstone , Canadian physiologist and poet
- Jim Johnstone , American baseball umpire
- Jim Johnstone , American basketball player
- Jimmy Johnstone, Scottish footballer
- James Johnstone , Scottish rugby union player