James Humphrey

• James Humphrey (New York politician), U.S. Representative from New York
• James Brown Humphrey, musician, bandleader, and music instructor in New Orleans, Louisiana
• James K. Humphrey, founder of the United Sabbath-Day Adventist Church, a small African American Christian denomination
• James M. Humphrey, also a U.S. Representative from New York
• James Humphrey (convict), Australian convict and schoolteacher
• James Ellis Humphrey, American botanist and mycologist
• James H. Humphrey, educator and leader in the field of physical education