Tetramethyl acetyloctahydronaphthalenes
Tetramethyl acetyloctahydronaphthalenes is a synthetic ketone fragrance also known as OTNE and by other commercial trade names such as: Iso E Super, Iso Gamma Super, Anthamber, Amber Fleur, Boisvelone, Iso Ambois, Amberlan, Iso Velvetone, Orbitone, Amberonne. It is a synthetic woody odorant and is used as a fragrance ingredient in perfumes, laundry products and cosmetics.
Odour
OTNE has a woody, slightly ambergris odour, reminiscent of clean human skin. Its odour is long-lasting on skin and fabric.Production
In commercial applications, Iso E Super is produced via a Diels–Alder reaction.Chemical Summary
OTNE is the abbreviation for the fragrance material with Chemical Abstract Service numbers 68155-66-8, 54464-57-2 and 68155-67-9 and EC List number 915-730-3. It is a multi-constituent isomer mixture containing:- 1-ethan-1-one
- 1-ethan-1-one
- 1-ethan-1-one
Physical-chemical properties
OTNE is a clear yellow liquid at 20 °C. Its melting point is below −20 °C at atmospheric pressure, and its boiling point is determined to be at around 290 °C. All physicochemical data have been obtained from the OTNE REACH registration dossier.Safety
Iso E Super may cause allergic reactions detectable by patch tests in humans and chronic exposure to Iso E Super from perfumes may result in permanent hypersensitivity. In a study with female mice, Iso E Super was positive in the local [lymph node assay] and irritancy assay, but negative in the mouse ear swelling test.No data were available regarding chemical disposition, metabolism, or toxicokinetics; acute, short-term, subchronic, or chronic toxicity; synergistic or antagonistic activity; reproductive or teratological effects; carcinogenicity; genotoxicity; or immunotoxicity.
The International Fragrance Association has published safe use levels for Iso E Super in consumer products.
OTNE is not toxic and not a CMR substance.
OTNE is classified as a skin irritant and is positive in the Local Lymph Node Assay and therefore classified as a skin sensitiser, though OTNE lacks any structural alerts for sensitisation in in silico prediction models and is not identified as an allergen in in vivo Human Repeated Patch Tests.
Several health related studies have been conducted on OTNE, and based on these studies, OTNE has been determined to be safe under the current conditions of use.
Given the sensitization classification of OTNE, and its use in fragrances, the International Fragrance Association has published safe use levels for OTNE in consumer products, which have been in effect since August 2009.
Environmental data
OTNE is classified as H410 Very toxic to aquatic life with long-lasting effects or R51/53 Toxic to aquatic organisms, may cause long-term adverse effects in the aquatic environment.The biodegradation of OTNE in fresh water is at most 40 days, and at most 120 days in sediment, though the biodegradation within the 28day window was around 11%. Given the outcome of the OECD 314 test OTNE does not meet the criteria for “Persistent” or “very Persistent”.
The measured Bio Concentration Factor is 391 L/kg, which is well below the EU limit of 2000 and US limit of 1000 for Bioaccumulation classification. The LogKow for OTNE has been measured to be 5.65.
OTNE is therefore not classified as a PBT or vPvB substance for the EU or any other global criteria.
OTNE has been detected in surface water at levels of 29–180 ng/L, These values are well below the Predicted No Effect Concentration and as a result the overall environmental risk ratio is determined to be below 1.
Regulatory status
OTNE is registered on all major chemical inventories and has been EU REACH registered in 2010.In 2014 the US National Toxicology Program conducted a 13-week repeat dose toxicity study and found no adverse effects.
OTNE has been recommended for inclusion in an update for the EU Fragrance Allergens labelling for cosmetic products based on a small number of positive reactions in dermatological clinics of around 0.2% to 1.7% of patients tested in three studies
If the proposed SCCS Opinion is taken forward into legislation then OTNE will be labelled on cosmetic products in the EU, several years after publication of a new legislation.
Commercial products
- The fragrance Molecule 01 is a specific isomer of Iso E Super, by the company IFF. Its partner fragrance Escentric 01 contains Iso E Super along with ambroxan, pink pepper, green lime with balsamic notes like benzoin mastic and incense.
- The fragrance Eternity by Calvin Klein contained 11.7% Iso E Super in the fragrance portion of the formula.
- The fragrance Scent of a Dream by Charlotte Tilbury contains Iso E Super.
- The fragrance No.1 Invisible by Perfume Extract contains Iso E Super.
- The fragrance I Don't Know What by D.S. & Durga leans heavily into Iso E Super.
- Most fragrances from the Danish brand Zarkoperfume contain Iso E Super.