Intrathoracic inoculation
Intrathoracic inoculation is a method of inoculation where a virus or other infectious agent is injected directly into the thorax of an insect, often mosquitoes, bypassing the midgut and other barriers to viral infection. This leads to a higher proportion of disseminated infections compared to blood feeding. This technique is used prevalently in Aedes spp., which are known to exhibit midgut infection and midgut escape barriers. The mosquito is therefore infected with a more constant dose of the virus.