John Gillespie
John Gillespie may refer to:
- John H. Gillespie, evolutionary biologist
- John Gillespie (auditor) state auditor and commissioner in Nebraska
- Dizzy Gillespie, jazz trumpeter
- Jimmy Gillespie, Scottish rugby union player
- John Ross Gillespie, field hockey player and coach from New Zealand
- John Gillespie (baseball), Major League Baseball pitcher
- John Gillespie (footballer, born 1870), Scottish footballer for Queen's Park FC and Scotland
- John Gillespie (footballer, born 1873), Scottish footballer for Sunderland
- John Gillespie (moderator), Moderator of the General Assembly of the Church of Scotland in 1903/04
- John Hamilton Gillespie, Scottish-American soldier, land developer, businessman and politician
- John Gillespie (legislator), member of the Wisconsin State Assembly
- John Gillespie (producer) of Hollywood North
- John Gillespie (Tennessee) Member of the Tennessee House of Representatives
- John Wynn Gillespie, American botanist whose standard author abbreviation is '''Gillespie'''