Gamma-150 RNA motif

The gamma-150 RNA motif is a conserved RNA structure that is found in bacteria within the order Pseudomonadales. Because gamma-150 RNAs are not consistently in 5' UTRs, the gamma-150 motif is presumed to correspond to a non-coding RNA.
Experiments conducted on RNA transcripts in Pseudomonas syringae DC3000 revealed that two gamma-150 RNAs in that organism are transcribed as separate RNA molecules. The transcript length is roughly 380 nucleotides in size, which is almost twice as large as the gamma-150 motif itself. It is likely that they are transcribed by RpoN, a protein that is also referred to as sigma 54.
Structurally, the gamma-150 motif consists of four independent stem-loops. The first stem-loop has significant covariation in support of its identity as an RNA. The second and fourth have more modest evidence, while the third stem-loop might be poorly conserved, or not genuine. Several regions of high conservation of nucleotide identity are present throughout the RNA motif, and many contain short runs of adenosines.