Gainesville Regional Transit System
Gainesville Regional Transit System is the local area transit corporation that serves the Gainesville, Alachua County, Florida area, the University of Florida and Santa Fe College campuses. It presently serves 40 city routes and 10 campus routes. Paratransit service is also provided to anyone with a Gainesville address.
City routes
| No. | Description | Operates |
| 1 | Rosa Parks RTS Downtown Station to Butler Plaza RTS Transfer Station | Mon-Sun |
| 2 | Rosa Parks RTS Downtown Station to NE Walmart Supercenter | Mon-Sat |
| 3 | Rosa Parks RTS Downtown Station to N Main St. Post Office | Mon-Fri |
| 5 | Rosa Parks RTS Downtown Station to [The The Oaks Mall (Florida)|Oaks Mall (Florida)|Oaks Mall] | Mon-Sun |
| 6 | Rosa Parks RTS Downtown Station to North Walmart Supercenter | Mon-Sat |
| 7 | Rosa Parks RTS Downtown Station to Eastwood Meadows | Mon-Fri |
| 8 | UF Health Shands Hospital to North Walmart Supercenter | Mon-Sun |
| 9 | Reitz Union to Hunters Run | Mon-Sun |
| 10 | Rosa Parks RTS Downtown Station to Santa Fe College | Mon-Sat |
| 11 | Rosa Parks RTS Downtown Station to Eastwood Meadows | Mon-Sun |
| 12 | Reitz Union to Butler Plaza RTS Transfer Station | Mon-Sun |
| 13 | Beaty Towers at the University of Florida to Cottage Grove Apartments | Mon-Sun |
| 15 | Rosa Parks RTS Downtown Station to NW 13th St./NW 23rd Ave. | Mon-Sun |
| 16 | Beaty Towers at the University of Florida to Sugar Hill | Mon-Sun |
| 17 | Beaty Towers at the University of Florida to Rosa Parks RTS Downtown Station | Mon-Fri |
| 20 | Reitz Union to Oaks Mall | Mon-Sun |
| 21 | Reitz Union to Cabana Beach Apts | Mon-Fri |
| 23 | Oaks Mall to Santa Fe College | Mon-Fri |
| 25 | UF Commuter Lot to Gainesville Regional Airport | Mon-Sun |
| 26 | Rosa Parks RTS Downtown Station to Gainesville Regional Airport | Mon-Fri |
| 27 | Rosa Parks RTS Downtown Station to NE Walmart Supercenter | Mon-Fri |
| 28 | The Hub to Butler Plaza RTS Transfer Station | Mon-Fri |
| 33 | Butler Plaza RTS Transfer Station to Midtown | Mon-Sun |
| 34 | The Hub to Lexington Crossing Apts | Mon-Fri |
| 35 | Reitz Union to SW 35th Place | Mon-Sun |
| 36 | The Hub to SW 34th St. Post Office | Mon-Fri |
| 37 | Reitz Union to Butler Plaza RTS Transfer Station | Mon-Sun |
| 38 | The Hub to Enclave Apts | Mon-Fri |
| 40 | The Hub to Hunters Crossing | Mon-Fri |
| 43 | UF Health Shands Hospital to Santa Fe College | Mon-Fri |
| 46 | Reitz Union to Rosa Parks RTS Downtown Station | Mon-Fri |
| 75 | Oaks Mall to Butler Plaza RTS Transfer Station | Mon-Sun |
| 76 | Haile Plantation to Santa Fe College | Mon-Fri |
| 78 | Butler Plaza RTS Transfer Station to Santa Fe College | Mon-Fri |
| 150 | Haile Plantation to UF Health Shands Hospital | Mon-Fri |
| 711 | Rosa Parks RTS Downtown Station to Eastwood Meadows | Mon-Fri after 8:00 p.m., all day Sat-Sun |
Campus Service
10 RTS routes operate on the University of Florida campus. These include "park and ride" and circulator routes. UF and Santa Fe College students have prepaid unlimited access to RTS services through a transportation fee attached to each credit hour for undergraduates. UF faculty, staff, spouses and retirees have unlimited prepaid access through the RTS Employee Bus Pass Program, as do SF faculty, staff and students.| No. | Description | Operates |
| 117 | Reitz Union to Park-N Ride 2 | Mon-Fri |
| 118 | The Hub to Park-N Ride 1 | Mon-Fri |
| 119 | The Hub to Family Housing at the University of Florida | Mon-Fri |
| 120 | The Hub to University of Florida West Circulator | Mon-Fri |
| 121 | The Hub to UF Commuter Lot | Mon-Fri |
| 122 | University of Florida North/South Circulator | Mon-Fri |
| 125 | The Hub to Lakeside | Mon-Fri |
| 126 | Sorority Row to Lakeside | Mon-Fri |
| 127 | University of Florida East Circulator | Mon-Fri |
| 128 | Reitz Union to Lake Wauberg | Saturdays during spring and the first half of summer |
Later Gator
Later Gator is a late night service that provides safe, late night service for students between campus, downtown, the Oaks Mall and southwest Gainesville through five routes. This service has been cancelled with no clear future plans to return service to the routes.| Route Letter | Description | Operates |
| A | Sorority Row to and from Downtown Station | 8:30pm – 3:20am Wednesday-Saturday |
| B | SW Gainesville to and from Downtown Station | 8:50pm – 3:13am Thursday-Saturday |
| C | Oaks Mall to and from Downtown Station | 8:30pm – 3:14am Thursday-Saturday |
| D | Cottage Grove Apts. to and from Downtown Station | 8:00pm – 2:30am Saturday Only |
| F | Butler Plaza to and from Downtown Station | 8:30pm – 3:18am Saturday Only |