Fast and Flexible Synthesis of Combinatorial Libraries for Directed Evolution
Fast and Flexible Synthesis of Combinatorial Libraries for Directed Evolution is a scholarly work by Neil Swainston and Lucy Green, published in 2018 in ''Methods in Enzymology''. The main subjects of the publication include directed evolution, ribosome, genetics, protein engineering, biology, DNA shuffling, shuffling, microfluidics, scope, selection (genetic algorithm), computer science, thermostability, computational biology, peptide synthesis, and mutagenesis. Knowledge of protein structure, dynamics, enzyme mechanisms, and natural evolution demonstrates that multiple (combinatorial) mutations are required to discover the most improved variants.