2000–01 Eerste Divisie
The Dutch Eerste Divisie in the 2000–01 season was contested by 18 teams. FC Den Bosch won the championship.
New entrants
Relegated from the 1999–2000 EredivisieLeague standings
Promotion/relegation play-offs
In the promotion/relegation competition, eight entrants entered in two groups. The group winners were promoted to the Eredivisie.Attendances
| # | Club | Average |
| 1 | Cambuur | 5,062 |
| 2 | Heracles | 4,672 |
| 3 | Emmen | 4,587 |
| 4 | Zwolle | 4,276 |
| 5 | Veendam | 4,028 |
| 6 | Go Ahead | 3,574 |
| 7 | Den Bosch | 3,333 |
| 8 | MVV | 2,762 |
| 9 | Volendam | 2,397 |
| 10 | Eindhoven | 2,342 |
| 11 | Helmond | 2,309 |
| 12 | ADO | 2,133 |
| 13 | Oss | 2,087 |
| 14 | Telstar | 1,875 |
| 15 | Excelsior | 1,794 |
| 16 | VVV | 1,780 |
| 17 | Haarlem | 1,522 |
| 18 | Dordrecht | 1,476 |
Source: