List of CSX Transportation lines

owns and operates a vast network of rail lines in the United States east of the Mississippi River. In addition to the major systems which merged to form CSX – the Baltimore and Ohio Railroad, Chesapeake and Ohio Railway, Louisville and Nashville Railroad, Atlantic Coast Line Railroad and Seaboard Air Line Railroad – it also owns major lines in the Northeastern United States acquired from the 1999 breakup of Conrail.
The lines are split into two regions – Northern and Southern, further split into divisions, and finally into subdivisions, most of which consist of a single main line with short branches.

Active lines

CSX Northern Region

[Albany Division]

!Subdivision
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!Notes
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Baltimore Division

!Subdivision
!From
!To
!Notes
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Chicago Division

!Subdivision
!From
!To
!Notes
!colspan=4|

Great Lakes Division

!Subdivision
!From
!To
!Notes
!colspan=4|

[Louisville Division]

!Subdivision
!From
!To
!Notes
!colspan=4|

CSX Southern Region

[Atlanta Division]

!Subdivision
!From
!To
!Notes
!colspan=4|

[Florence Division]

!Subdivision
!From
!To
!Notes
!colspan=4|

[Jacksonville Division]

!Subdivision
!From
!To
!Notes
!colspan=4|

[Nashville Division]

!Subdivision
!From
!To
!Notes
!colspan=4|

Former lines

Northern Region

Albany Division

!Subdivision
!From
!To
!Reason
!colspan=4|

Baltimore Division

!Subdivision
!From
!To
!Notes
!colspan=4|

Chicago Division

!Subdivision
!From
!To
!Notes
!colspan=4|

Great Lakes Division

!Subdivision
!From
!To
!Notes
!colspan=4|

Louisville Division

!Subdivision
!From
!To
!Notes
!colspan=4|

Southern Region

[Atlanta Division]

!Subdivision
!From
!To
!Notes
!colspan=4|

Florence Division

!Subdivision
!From
!To
!Notes
!colspan=4|

Monongah Division

!Subdivision
!From
!To
!Notes
!colspan=4|

Nashville Division

!Subdivision
!From
!To
!Notes
!colspan=4|

Huntington Division

!Subdivision
!From
!To
!Notes
!colspan=4|

Appalachian Division

!Subdivision
!From
!To
!Notes