Coprophanaeus ensifer
Coprophanaeus ensifer is a large South American species of beetle belonging to the family Scarabaeidae. This species is necrophagous and builds burrows near or on animal carcasses to dismember the flesh of decaying bodies and bring it to its burrow to feed. Both females and males help build the burrow and feed. It is characterized by its iridescent colors and a horn that is similar in shape and size in females and males. It uses its horn to tear apart carcasses and to fight with other individuals, with male-male fighting occurring more often. However, females also fight to determine a variety of characteristics of the opposing male. This species may be of importance in forensic science due to its destructive behavior on decaying bodies, especially in areas of Brazil where homicide rates are high.
Phylogeny
C. ensifer vary in color and distribution, which has created uncertainty and barriers in its taxonomy within the Coprophanaeus genus. Based on its mitogenome, the species was placed in the Phaneini tribe, which forms a clade of Scarabaeinae with other tribes like the Coprini tribe. Among species in this clade, most reside in South America except for Sarophorus sp., which is found in South Africa. Based on this, researchers have suggested Scarabaeinae beetles came to South America from Africa through colonization of the common ancestor of Sarophorus sp. and species in the clade with ''C. ensifer.''Distribution and habitat
This species is found widely in eastern, central and southern Brazil, far northeastern Argentina, eastern Bolivia and Paraguay, occurring up to about above sea level. Most of its range is in the Caatinga, Cerrado and similar habitats, but in some regions its range also extends into the Atlantic forest and the Amazon rainforest.A relates species, C. lancifer occupies northern Brazil, limiting the distribution of C. ensifer in those regions. C. lancifer was also thought to occupy the Juína region, but upon genetic investigation, it was found that individuals in that region are more closely related to C. ensifer, suggesting that the distribution of C. ensifer is wider than previously thought.
A study found that in Northeast Brazil, C. ensifer is more active during the rainy season, when temperatures drop. This preference can be explained by their large body. At high temperatures during dry seasons, it could be difficult to maintain a proper water balance because a large amount of water can be evaporated from their bodies. This could also explain why these beetles are crepuscular, or most active at dawn and dusk.
Building burrows
In many cases, both females and males work together to build a burrow. Pairs that build burrows together are usually larger than other conspecifics. Females mainly built the burrow by pushing soil while males bring the soil up to the surface. The burrow is usually built near a carcass so that females and males can break it down to bring it inside the burrow by rolling the pieces of food. Cooperation between males and females suggests that parental care is offered by both sexes.In other cases, females and males build burrows individually despite having mated with other individuals. Occasionally, small individuals build burrows next to burrows built by a pair.
Although the effects of bioturbation, or moving and mixing soil, caused by building burrows has not been empirically studied, it has been suggested that this could have an effect on the soil itself by increasing aeration and the ability for water to penetrate.
Diet
Compared to other Scarabaeidae species who are coprophagous, this species is necrophagous, which means it feeds on carrion, or the decaying body of dead animals. It has even been reported to bury a dead snake to feed on it. A carcass goes through various stages of decomposition and C. ensifer is most attracted to the body when it is actively decomposing in the initial stages of decomposition.By breaking down carrion and moving it or burying it, nutrient cycling may occur where nutrients that were part of the decaying carcass reenter the soil, affecting plant growth.
It has been suggested that the difference in diet in C. ensifer compared to other species in the Scarabaeidae family evolved in the Pleistocene because of the extinction of large mammals, so the beetles were forced to change their diet to decrease competition and ensure survival.
These beetles find decomposing carcasses through the detection of volatiles released during the decomposition process. A study found that females always arrived first to a pig carcass after the sun set, while males arrived later. Once the beetles arrive at the carcass, males bury themselves while females feed from it using their horn. The difference in arrival time suggest that females first find a suitable resource to feed and for oviposition while males arrive later to mate with females. Another potential explanation could be that males take advantage of females who have started building burrows, so that they can avoid digging tunnels.
Fighting behavior
Both females and males engage in fighting activity using their horns. There can be male-male, female-female, or male-female fighting in these beetles. In burrows, individuals use their horn to carry and drop the other but on the surface of burrows, individuals fight by pushing each other. Males are more aggressive than females.Female fighting against males may simply occur to refuse copulation after they have already copulated. However, another suggestion is that fighting can be used as a way to determine mates. If horns act as an ornament to attract females, fighting could be a way for females to gauge which males to mate with. If females can also decide which sperm to use to fertilize their eggs, engaging in fights after copulation could also serve to detect which sperm to use. When males fight, the larger male always wins, so this may also be a mechanism for mate choice. Lastly, males may also determine which female to mate with based on fighting. Larger females may be more beneficial to mate with because they can protect burrows, so fighting can be a way for males to determine female size.
Genetics
The size of the mitogenome of C. ensifer is 14,964 bp organized into rRNA, tRNA, and protein coding genes, which is similar to species in the Coleptera order. C. ensifer, along most of Scarabaeinae species, are diploid. This species specifically, has a XY sex-determining mechanism where the X and Y chromosomes do not exhibit any different configurations observed in other species.Copulation behavior
Four stages of copulation have been defined for Neotropical Scarabaeinae beetles. The first stage involves the male approaching the female, which is usually around a carcass or burrow. In the second stage, the male detects the female. The third stage involves the male mounting on the female. It has been observed that while the mounting occurs, the male strokes the abdominal tergum with his legs so that the female can reveal her genital opening. During the last stage, the males insert their aedeagus in the female genital opening.While all males attempt to mate with females, larger males are more successful and among males, copulations are more successful when attempting with a smaller female. Copulation activity mainly takes place underground in burrows and tunnels and lasts around one to two minutes.