Cap binding complexThe 5' cap of eukaryotic messenger RNA is bound at all times by various cap-binding complexes.Nuclear cap-binding complexIn the nucleus, freshly transcribed mRNA molecules are bound on the 5' cap by the nuclear cap-binding complex of Cbc1/Cbc2 in yeast or CBP20/CBP80 in metazoans. These aid in the export of the mRNA and protect it from decapping. They also serve as a marker for the so-called pioneer round of translation when the message is examined by nonsense mediated decay.Cytoplasmic cap-binding complexAfter the first round of translation, CBC20/80 is replaced by the translation initiation factor eIF4E. The eIF4F complex then regulates translation in response to the state of the cell via its phosphorylation state and again protects the message from decapping.Decapping complexWhen translationally repressed or marked for decay by various mechanisms the 5' cap is bound by the mRNA decapping enzyme DCP2. A host of proteins accompany it including UPF1, UPF2, UPF3A, Dcp1, Dhh1, XRN1, and others. The decapping enzyme removes the 5' cap leading to destruction of the message.