Blood–testis barrier
The blood–testis barrier is a physical barrier between the blood vessels and the seminiferous tubules of the animal testes. The name "blood-testis barrier" is misleading as it is not a blood-organ barrier in a strict sense, but is formed between Sertoli cells of the seminiferous tubule and isolates the further developed stages of germ cells from the blood. A more correct term is the Sertoli cell barrier.
Structure
The walls of seminiferous tubules are lined with primitive germ layer cells and by Sertoli cells. The barrier is formed by tight junctions, adherens junctions and gap junctions between the Sertoli cells, which are sustentacular cells of the seminiferous tubules, and divides the seminiferous tubule into a basal compartment and an endoluminal compartment. The tight junctions are formed by intercellular adhesion molecules in between cells that are anchored to actin fibers within the cells. For the visualization of the actin fibers within the seminiferous tubules see Sharma et al.'s immunofluorescence studies.Function
The presence of the SCB allows Sertoli cells to control the adluminal environment in which germ cells develop by influencing the chemical composition of the luminal fluid.The barrier also prevents passage of cytotoxic agents into the seminiferous tubules.
The fluid in the lumen of seminiferous tubules is quite different from plasma; it contains very little protein and glucose but is rich in androgens, estrogens, potassium, inositol and glutamic and aspartic acid. This composition is maintained by blood–testis barrier.
The barrier also protects the germ cells from blood-borne noxious agents,
prevents antigenic products of germ cell maturation from entering the circulation and generating an autoimmune response, and may help establish an osmotic gradient that facilitates movement of fluid into the tubular lumen.
Note
Steroids penetrate the barrier.Some proteins pass from Sertoli cells to Leydig cells to function in a paracrine fashion.