Asymmetric PCR
Asymmetric PCR is a variation of PCR used to preferentially amplify one strand of the original DNA more than the other. The technique has applications in some types of sequencing and hybridization probing where having only one of the two complementary strands is required.
Methodology
Asymmetric PCR differs from regular PCR by the excessive amount of primers for a chosen strand. Due to the slow amplification later in the reaction extra cycles of PCR are required.A modification on this process, known as Linear-After-The-Exponential-PCR, uses a limiting primer with a higher melting temperature than the excess primer to maintain reaction efficiency as the limiting primer concentration decreases mid-reaction.