AIS
- AI safety
- AIS Airlines
- Abbreviated Injury Scale
- Abu Dhabi International School
- Accounting information system
- Advanced Info Service
- Aeronautical Information Service
- Agnes Irwin School
- Ahmadhiyya International School
- Ais (mythology)
- Ais people
- Alarm indication signal
- Alarm indication signal line
- Alarm indication signal path
- Almaty International School
- Alternate Instruction Set
- American Indycar Series
- American International School (disambiguation)
- Androgen insensitivity syndrome
- Antonine International School
- Antwerp International School
- Application Interface Specification
- Arorae Island Airport
- Artificial Intelligence System
- Artificial immune system
- Asahi India Glass Limited
- Association for Information Systems
- Athens Insomnia Scale
- Atlanta International School
- Australian Information Service
- Australian Institute of Sport
- Australian International School (disambiguation)
- Automated information system
- Automatic identification system
- Automotive Industry Standards
- Aviatsionnaya Ispitatelnaya Stantsiya
- Eyeish
- International Academy of Sciences San Marino
- Islamic Salvation Front