AFO
- A Funk Odyssey
- AFO Records
- Advanced Force Operations
- Aero Force One
- Afghan Film Organization
- Afo Dodoo
- Afton Municipal Airport
- Air Force One (disambiguation)
- Alien Front Online
- Ameloblastic fibro-odontoma
- Animal Face-Off
- Animal feeding operation
- Anime Festival Orlando
- Ankle-foot orthosis
- Anti-Fascist Organisation
- Arellano-Felix Organization
- Assemblée de la francophonie de l'Ontario
- Association of Field Ornithologists
- Australian Field Ornithology
- Authorised Firearms Officer
- Clove Tree
- Eloyi language
- Eloyi people