ANNINE-6plus

ANNINE-6plus is a water soluble voltage sensitive dye. This compound was developed at the Max Planck Institute for Biochemistry in Germany. It is used to optically measure the changes in transmembrane voltage of excitable cells, including neurons, skeletal and cardiac myocytes.

Voltage sensitivity

ANNINE-6plus has a fractional fluorescent intensity change of about 30% with single-photon excitation and >50% with two-photon excitation.

Applications

ANNINE-6plus has been applied in the microscopic imaging of action potentials of cardiomyocyte in perfused mice heart. Using confocal microscopy in conjunction with ANNINE-6plus, single sweep action potentials with high peak signal-to-noise ratio have been recorded from single transverse tubule of a few micrometers in the ventricular cardiomyocyte.