| Member | Electoral district | Party | First elected / previously elected | No.# of term | Notes
| John Cobb | Arthur | Progressive Conservative | 1958 | 2nd term | Died in office August 21, 1959
| Douglas Watt | 1959 | 1st term | From November 26, 1959
| George William Johnson | Assiniboia | Progressive Conservative | 1959 | 1st term
| Robert Smellie | Birtle-Russell | Progressive Conservative | 1959 | 1st term
| Reginald Lissaman | Brandon | Progressive Conservative | 1952 | 4th term
| Edward Schreyer | Brokenhead | CCF | 1958 | 2nd term
| John Hawryluk | Burrows | CCF | 1958 | 2nd term
| Edmond Prefontaine | Carillon | Liberal-Progressive | 1935 | 8th term
| John Ingebrigtson | Churchill | Progressive Conservative | 1959 | 1st term
| Marcel Boulic | Cypress | Progressive Conservative | 1958 | 2nd term | Died in office September 22, 1959
| Thelma Forbes | 1959 | 1st term | From November 26, 1959
| Stewart McLean | Dauphin | Progressive Conservative | 1958 | 2nd term
| William Homer Hamilton | Dufferin | Progressive Conservative | 1959 | 1st term
| Steve Peters | Elmwood | CCF | 1958 | 2nd term
| John Tanchak | Emerson | Liberal-Progressive | 1957 | 3rd term
| Michael Hryhorczuk | Ethelbert Plains | Liberal-Progressive | 1949 | 4th term
| Peter Wagner | Fisher | CCF | 1958 | 2nd term
| Charles Witney | Flin Flon | Progressive Conservative | 1959 | 1st term
| Sterling Lyon | Fort Garry | Progressive Conservative | 1958 | 2nd term
| Gurney Evans | Fort Rouge | Progressive Conservative | 1953 | 3rd term
| George Johnson | Gimli | Progressive Conservative | 1958 | 2nd term
| Nelson Shoemaker | Gladstone | Liberal-Progressive | 1958 | 2nd term
| Barry Strickland | Hamiota | Progressive Conservative | 1958 | 2nd term
| Morris Gray | Inkster | CCF | 1941 | 6th term
| Anthony J. Reid | Kildonan | CCF | 1958 | 2nd term
| Oscar Bjornson | Lac du Bonnet | Progressive Conservative | 1959 | 1st term
| Douglas Lloyd Campbell | Lakeside | Liberal-Progressive | 1922 | 10th term
| Stan Roberts | La Verendrye | Liberal-Progressive | 1958 | 2nd term
| Lemuel Harris | Logan | CCF | 1959 | 1st term
| Walter Weir | Minnedosa | Progressive Conservative | 1959 | 1st term
| Harold Shewman | Morris | Progressive Conservative | 1949 | 4th term
| Obie Baizley | Osborne | Progressive Conservative | 1959 | 1st term
| Maurice Ridley | Pembina | Progressive Conservative | 1957 | 3rd term | Died in office October 2, 1960
| Carolyne Morrison | 1960 | 1st term | From December 9, 1960
| John Christianson | Portage la Prairie | Progressive Conservative | 1959 | 1st term
| Russell Paulley | Radisson | CCF | 1953 | 3rd term
| Wallace C. Miller | Rhineland | Liberal-Progressive | 1936 | 7th term | Died in office October 4, 1959
| Jacob Froese | Social Credit | 1959 | 1st term | From November 26, 1959
| William B. Scarth | River Heights | Progressive Conservative | 1958 | 2nd term
| Keith Alexander | Roblin | Progressive Conservative | 1958 | 2nd term
| Abram Harrison | Rock Lake | Progressive Conservative | 1943 | 6th term
| George Hutton | Rockwood—Iberville | Progressive Conservative | 1959 | 1st term
| Joseph Jeannotte | Rupertsland | Progressive Conservative | 1958 | 2nd term
| Laurent Desjardins | St. Boniface | Liberal-Progressive | 1959 | 1st term
| Elman Guttormson | St. George | Liberal-Progressive | 1956 | 3rd term
| Douglas Stanes | St. James | Progressive Conservative | 1958 | 2nd term
| David Orlikow | St. Johns | CCF | 1958 | 2nd term
| William G. Martin | St. Matthews | Progressive Conservative | 1958 | 2nd term
| Fred Groves | St. Vital | Progressive Conservative | 1958 | 2nd term
| Gildas Molgat | Ste. Rose | Liberal-Progressive | 1953 | 3rd term
| Thomas P. Hillhouse | Selkirk | Liberal-Progressive | 1950 | 4th term
| Arthur E. Wright | Seven Oaks | CCF | 1958 | 2nd term
| Malcolm Earl McKellar | Souris-Lansdowne | Progressive Conservative | 1958 | 2nd term
| Fred Klym | Springfield | Progressive Conservative | 1959 | 1st term
| Albert H. C. Corbett | Swan River | Progressive Conservative | 1958 | 2nd term
| John Carroll | The Pas | Progressive Conservative | 1958 | 2nd term
| Errick Willis | Turtle Mountain | Progressive Conservative | 1936 | 7th term | Named Lieutenant Governor and resigned seat but remained in Cabinet until January 15, 1960
| Edward Dow | Liberal-Progressive | 1959 | 1st term | From November 26, 1959
| John Thompson | Virden | Progressive Conservative | 1953 | 3rd term
| Richard Seaborn | Wellington | Progressive Conservative | 1958 | 2nd term
| James Cowan | Winnipeg Centre | Progressive Conservative | 1958 | 2nd term
| Dufferin Roblin | Wolseley | Progressive Conservative | 1949 | 4th term |