2025 Italian Open – Women's singles
Jasmine Paolini defeated Coco Gauff in the final, 6–4, 6–2 to win the women's singles tennis title at the 2025 Italian Open. It was her second WTA 1000 and third career WTA Tour singles title. Paolini was the second Italian woman in the Open Era to win the Italian Open singles title, after Raffaella Reggi in 1985. The final was also a rematch of the doubles final from the previous year. Paolini was the first Italian woman to win both the singles and doubles titles at the tournament in the same year since Reggi in 1985, and the first woman to do so in Rome since Monica Seles in 1990 and overall since Vera Zvonareva in 2009.
Iga Świątek was the defending champion, but lost in the third round to Danielle Collins.
Gauff was the seventh and youngest woman to reach the finals of both the Madrid and Italian Opens in the same year. Peyton Stearns became the first player in the Open Era to win three straight WTA Tour matches in third-set tiebreakers, against Madison Keys, Naomi Osaka, and Elina Svitolina in the third round, fourth round, and quarterfinals respectively.
Seeds
All seeds received a bye into the second round.Draw
Finals
Seeded players
The following are the seeded players. Seedings are based on WTA rankings as of 21 April 2025. Rankings and points before are as of 5 May 2025.Under the 2025 Rulebook, points from six of the seven combined WTA 1000 tournaments are required to be counted in a player's ranking.
| Seed | Rank | Player | Points before | Points defending | Points earned | Points after | Status | ||||||||
| 1 | 1 | Aryna Sabalenka | 11,118 | 650 | 215 | 10,683 | Quarterfinals lost to  Withdrawn seeded playersThe following player would have been seeded, but withdrew before the tournament began.
|
