2000 CIAU University Cup
The 2000 CIAU Men's University Cup Hockey Tournament was held at the Rutherford Arena in Saskatoon, Saskatchewan. The Saskatchewan Huskies served as tournament host.
Road to the Cup
AUS playoffs">AUS men's ice hockey tournament#2000">AUS playoffs
Note: * denotes overtime periodOUA playoffs">OUA men's ice hockey tournament#2000">OUA playoffs
Note: * denotes overtime periodCanada West playoffs">Canada West men's ice hockey tournament#2000">Canada West playoffs
Note: * denotes overtime periodUniversity Cup
The rotating wild-card moved to Canada West. Due to the conference already advancing two teams due to Saskatchewan acting as host and participating in the conference championship, a third place game was instituted to determine the wild-card qualifier.All teams were ranked by committee with a preference to avoid intra-conference matches.
| Seed | Team | Qualification | Record | Appearance | Last |
| 1 | [Saskatchewan Huskies men's ice hockey|] | West: Canada West Champion / Host | 26–4–3 | 8th | 1999 |
| 2 | [UQTR Patriotes men's ice hockey|] | Quebec: OUA Champion | 24–3–4 | 10th | 1999 |
| 3 | [Alberta Golden Bears men's ice hockey|] | Wild-card: Canada West Runner-up | 23–5–5 | 25th | 1999 |
| 4 | [Western Mustangs men's ice hockey|] | Ontario: OUA Runner-up | 22–4–4 | 5th | 1995 |
| 5 | [UNB Reds men's ice hockey|] | Atlantic: AUS Champion | 22–13–0 | 6th | 1998 |
| 6 | [Calgary Dinos men's ice hockey|] | Wild-card: Canada West Third place | 20–11–3 | 10th | 1996 |
Bracket
Note: * denotes overtime periodNote: round-robin games were played on consecutive days March 23–25