Skin flora


Skin flora, also called skin microbiota, refers to microbiota that reside on the skin, typically human skin.
Many of them are bacteria of which there are around 1,000 species upon human skin from nineteen phyla. Most are found in the superficial layers of the epidermis and the upper parts of hair follicles.
Skin flora is usually non-pathogenic, and either commensal or mutualistic. The benefits bacteria can offer include preventing transient pathogenic organisms from colonizing the skin surface, either by competing for nutrients, secreting chemicals against them, or stimulating the skin's immune system. However, resident microbes can cause skin diseases and enter the blood system, creating life-threatening diseases, particularly in immunosuppressed people.
A major non-human skin flora is Batrachochytrium dendrobatidis, a chytrid and non-hyphal zoosporic fungus that causes chytridiomycosis, an infectious disease thought to be responsible for the decline in amphibian populations.

Species variety

Bacteria

The estimate of the number of bacteria species present on skin has been radically changed by the use of 16S ribosomal RNA to identify bacterial species present on skin samples direct from their genetic material. Previously such identification had depended upon microbiological culture upon which many varieties of bacteria did not grow and so were hidden to science.
Staphylococcus epidermidis and Staphylococcus aureus were thought from cultural based research to be dominant. However 16S ribosomal RNA research finds that while common, these species make up only 5% of skin bacteria. However, skin variety provides a rich and diverse habitat for bacteria. Most come from four phyla: Actinomycetota, Bacillota, Pseudomonadota, and Bacteroidota.
There are three main ecological areas: sebaceous, moist, and dry. Propionibacteria and Staphylococci species were the main species in sebaceous areas. In moist places on the body Corynebacteria together with Staphylococci dominate. In dry areas, there is a mixture of species but Betaproteobacteria and Flavobacteriales are dominant. Ecologically, sebaceous areas had greater species richness than moist and dry ones. The areas with least similarity between people in species were the spaces between fingers, the spaces between toes, axillae, and umbilical cord stump. Most similarly were beside the nostril, nares, and on the back.

Fungal

A study of the area between toes in 100 young adults found 14 different genera of fungi. These include yeasts such as Candida albicans, Rhodotorula rubra, Torulopsis and Trichosporon cutaneum, dermatophytes such as Microsporum gypseum, and Trichophyton rubrum and nondermatophyte fungi such as Rhizopus stolonifer, Trichosporon cutaneum, Fusarium, Scopulariopsis brevicaulis, Curvularia, Alternaria alternata, Paecilomyces, Aspergillus flavus and Penicillium species.
A study by the National Human Genome Research Institute in Bethesda, Maryland, researched the DNA of human skin fungi at 14 different locations on the body. These were the ear canal, between the eyebrows, the back of the head, behind the ear, the heel, toenails, between the toes, forearm, back, groin, nostrils, chest, palm, and the crook of the elbow. The study showed a large fungal diversity across the body, the richest habitat being the heel, which hosts about 80 species of fungi. By way of contrast, there are some 60 species in toenail clippings and 40 between the toes. Other rich areas are the palm, forearm and inside the elbow, with from 18 to 32 species. The head and the trunk hosted between 2 and 10 each.

Umbilical microbiome

The umbilicus, or navel, is an area of the body that is rarely exposed to UV light, soaps, or bodily secretions and because it is an almost undisturbed community of bacteria it is an excellent part of the skin microbiome to study. The navel, or umbilicus is a moist microbiome of the body, that contains a large amount of bacteria, especially bacteria that favors moist conditions such as Corynebacterium and Staphylococcus.
The Belly Button Biodiversity Project began at North Carolina State University in early 2011 with two initial groups of 35 and 25 volunteers. Volunteers were given sterile cotton swabs and were asked to insert the cotton swabs into their navels, to turn the cotton swab around three times and then return the cotton swab to the researchers in a vial that contained a 0.5 ml 10% phosphate saline buffer. Researchers at North Carolina State University, led by Jiri Hulcr, then grew the samples in a culture until the bacterial colonies were large enough to be photographed and then these pictures were posted on the Belly Button Biodiversity Project's website. These samples then were analyzed using 16S rDNA libraries so that strains that did not grow well in cultures could be identified.
The researchers at North Carolina State University discovered that while it was difficult to predict every strain of bacteria in the microbiome of the navel that they could predict which strains would be prevalent and which strains of bacteria would be quite rare in the microbiome. It was found that the navel microbiomes only contained a few prevalent types of bacteria and many different types of rare bacteria. Other types of rare organisms were discovered inside the navels of the volunteers including three types of Archaea, two of which were found in one volunteer who claimed not to have bathed or showered for many years.
Staphylococcus and Corynebacterium were among the most common types of bacteria found in the navels of this project's volunteers and these types of bacteria have been found to be the most common types of bacteria found on the human skin in larger studies of the skin microbiome.
According to the Belly Button Biodiversity Project at North Carolina State University, there are two types of microorganisms found in the navel and surrounding areas. Transient bacteria forms the majority of the organisms found in the navel, and an estimated 1400 various strains were found in 95% of participants of the study.
The Belly Button Biodiversity Project is ongoing and has now taken swabs from over 500 people. The project was designed with the aim of countering that misconception that bacteria are always harmful to humans and that humans are at war with bacteria. In actuality, most strains of bacteria are harmless if not beneficial for the human body. Another of the project's goals is to foster public interest in microbiology. Working in concert with the Human Microbiome Project, the Belly Button Biodiversity Project also studies the connections between human microbiomes and the factors of age, sex, ethnicity, location and overall health.

Relationship to host

Skin microflora can be commensals, mutualistic or pathogens. Often they can be all three depending upon the strength of the person's immune system. Research upon the immune system in the gut and lungs has shown that microflora aids immunity development: however such research has only started upon whether this is the case with the skin. Pseudomonas aeruginosa is an example of a mutualistic bacterium that can turn into a pathogen and cause disease: if it gains entry into the circulatory system it can result in infections in bone, joint, gastrointestinal, and respiratory systems. It can also cause dermatitis. However, P. aeruginosa produces antimicrobial substances such as pseudomonic acid. This works against staphylococcal and streptococcal infections. P. aeruginosa also produces substances that inhibit the growth of fungus species such as Candida krusei, Candida albicans, Torulopsis glabrata, Saccharomyces cerevisiae and Aspergillus fumigatus. It can also inhibit the growth of Helicobacter pylori. So important is its antimicrobial actions that it has been noted that "removing P. aeruginosa from the skin, through use of oral or topical antibiotics, may inversely allow for aberrant yeast colonization and infection."
Another aspect of bacteria is the generation of body odor. Sweat is odorless however several bacteria may consume it and create byproducts which may be considered putrid by humans.
Several examples are:

Antimicrobial peptides

The skin creates antimicrobial peptides such as cathelicidins that control the proliferation of skin microbes. Cathelicidins not only reduce microbe numbers directly but also cause the secretion of cytokine release which induces inflammation, angiogenesis, and reepithelialization. Conditions such as atopic dermatitis have been linked to the suppression in cathelicidin production. In rosacea abnormal processing of cathelicidin cause inflammation. Psoriasis has been linked to self-DNA created from cathelicidin peptides that causes autoinflammation. A major factor controlling cathelicidin is vitamin D3.

Acidity

The superficial layers of the skin are naturally acidic due to lactic acid in sweat and produced by skin bacteria. At this pH mutualistic flora such as Staphylococci, Micrococci, Corynebacterium and Propionibacteria grow but not transient bacteria such as Gram-negative bacteria like Escherichia and Pseudomonas or Gram positive ones such as Staphylococcus aureus. Another factor affecting the growth of pathological bacteria is that the antimicrobial substances secreted by the skin are enhanced in acidic conditions. In alkaline conditions, bacteria cease to be attached to the skin and are more readily shed. It has been observed that the skin also swells under alkaline conditions and opens up allowing bacterial movement to the surface.