Mole salamander

The mole salamanders are a group of advanced salamanders endemic to North America. The group has become famous due to the study of the axolotl in research on paedomorphosis, and the tiger salamander which is often sold as a pet, and is the official amphibian of four US states.

General description

mole salamanders are identified by having wide, protruding eyes, prominent costal grooves, and thick arms. Most have vivid patterning on dark backgrounds, with marks ranging from deep blue spots to large yellow bars depending on the species. Terrestrial adults spend most of their lives underground in burrows, either of their own making or abandoned by other animals. Some northern species may hibernate in these burrows throughout the winter. They live alone and feed on any available invertebrate. Adults spend little time in the water, only returning to the ponds of their birth to breed.
All mole salamanders are oviparous and lay large eggs in clumps in the water. Their fully aquatic larvae are branchiate, with three pairs of external gills behind their heads and above their gill slits. Larvae have large caudal fins, which extend from the back of their heads to their tails and to their cloacae. Larvae grow limbs soon after hatching, with four toes on the fore arms, and five toes on the hind legs. Their eyes are wide-set and lack true eyelids.
The larvae of some species can reach their adult size before undergoing metamorphosis. During metamorphosis, the gills of the larvae disappear, as do the fins. Their tails, skin, and limbs become thicker, and the eyes develop lids. Their lungs become fully developed, allowing for a fully terrestrial existence.
Some species of mole salamanders are neotenic. The most famous example is the axolotl. They cannot produce thyroxine, so their only means of metamorphosis is mainly through the outside injection of it. This usually shortens the lifespan of the salamander.

Tiger salamander complex

Morphologically, tiger salamanders have large heads, small eyes, and thick bodies. This basic morphology is similar across most mole salamanders, though tiger salamanders are among the largest of the mole salamanders, and have relatively large larvae.
Tiger salamanders inhabit a wide variety of ecosystems across North America. Given this geographic diversity, subpopulations of tiger salamanders exhibit morphological and behavioral diversity. Whether subpopulations constitute independent species or subspecies within the Ambystoma tigrinum complex, as well as the driving forces behind diversification, remains an active area of research as of 2024. Several subspecies within the Ambystoma tigrinum complex have been reclassified as an independent species. For example:

Ambystoma mavortium comprises former subspecies A. t. diaboli, A. t. mavortium, A. t. melanostictum, A. t. nebulosum, and A. t. stebbinsi.
Ambystoma californiense
Ambystoma velasci, which may be paraphyletic and shares habitats with axolotl
Hybrid all-female populations

Unisexual populations of ambystomatid salamanders are widely distributed across the Great Lakes region and northeastern North America. The females require sperm from a co‑occurring, related species to fertilize their eggs and initiate development. Usually the eggs then discard the sperm genome and develop asexually ; however, they may incorporate the genome from the sperm into the resulting offspring. Sperm incorporation commonly takes the form of genome addition, or genome replacement, wherein one of the maternal genomes is discarded. This unique mode of reproduction has been termed kleptogenesis by Bogart and colleagues. This is in contrast to hybridogenesis, where the maternal genomes are passed hemiclonally and the paternal genome is discarded every generation before the egg matures and reacquired from the sperm of another species.
The nuclear DNA of the unisexuals generally comprises genomes from up to five species: the blue-spotted salamander, Jefferson salamander, small-mouthed salamander, streamside salamander, and tiger salamander, denoted respectively as L, J, Tx, B, and Ti. This flexibility results in a large number of possible nuclear biotypes in the unisexuals. For example, an LJJ individual would be a triploid with one A. laterale genome and two A. jeffersonianum genomes, while an LTxJTi individual would be a tetraploid with genomes from four species. Because they have hybrid genomes, unisexual salamanders are a cryptic species with morphology similar to coexisting species. For example, LLJs look like blue-spotted salamanders and LJJs look like Jefferson salamanders. Silvery salamanders LJJ, Tremblay's salamanders LLJ, and Kelly's Island salamanders LTxTx and LTxTi were initially described as species. Species names were later dropped for all unisexual salamanders because of the complexity of their genomes. The offspring of a single mother may have different genome complements; for example, a single egg mass may have both LLJJ and LJJ larvae.
Despite the complexity of the nuclear genome, all unisexuals form a monophyletic group based on their mitochondrial DNA. The maternal ancestor of the unisexual ambystomatids was most closely related to the streamside salamander, with the original hybridization likely occurring 2.4~3.9 million years ago, making it the oldest known lineage of all-female vertebrates. The hybridization was most probably with an A. laterale. All known unisexuals have at least one A. laterale genome and this is thought to be essential for unisexuality. However, the A. laterale genome has been replaced several times, independently, in each of the lineages by matings with A. laterale.

Limb regeneration

Ambystoma mexicanum, a neotenic salamander with exceptional regenerative capabilities is one of the principal models for studying limb regeneration. Limb regeneration involves the propagation of a mass of low differentiated and highly proliferative cells termed the blastema. During limb regeneration, blastema cells experience DNA double-strand breaks and thus require homologous recombination, a form of DNA repair that deals with double-strand breaks.

Taxonomy

Rhyacosiredon was previously considered a separate genus within the family Ambystomatidae. However, cladistic analysis of the mole salamanders found the existence of Rhyacosiredon makes Ambystoma paraphyletic, since the species are more closely related to some Ambystoma species than those species are to others in Ambystoma. The stream-type morphology of these salamanders may have led to their misclassification as a different genus.
The genus name Ambystoma was given by Johann Jakob von Tschudi in 1839. Tschudi did not provide a derivation for the name, and many thought that he intended the name Amblystoma, "blunt-mouth". Occasionally, old specimens and documents use the name Amblystoma. Writing in 1907, Leonhard Stejneger offered a derivation of Ambystoma based on a contraction of a Greek phrase meaning "to cram into the mouth", but others have not found this explanation convincing. In the absence of clear evidence that Tschudi committed a lapsus, the name given in 1839 stands.

Species

The genus Ambystoma contains 32 species, listed below, the newest being A. bishopi. Some species are Terrestrial, others are neotenic, and some species have established populations of both neotenic and terrestrial forms.

Image	Species and author	Common name	Distribution	Lifestyle	IUCN status
	A. altamirani Dugès, 1895	Mountain stream salamander, Achoque	Central Mexico, west and south of the Valley of Mexico	Terrestrial and neotenic
	A. amblycephalum Taylor, 1940	Blunt-headed salamander	West-central Mexico, near Morelia	Terrestrial and neotenic
	A. andersoni	Anderson's salamander	West-central Mexico, Laguna de Zacapu	Neotenic
	A. annulatum Cope, 1886	Ringed salamander	South-central United States, Ozark Plateau and Ouachita Mountains	Terrestrial
	A. barbouri Kraus & Petranka, 1989	Streamside salamander	South-midwest United States	Terrestrial
	A. bishopi Pauly, Piskurek & Shaffer, 2007	Reticulated flatwoods salamander	Southeast United States, west of the Apalachicola-Flint River	Terrestrial
	A. bombypellum	Delicate-skinned salamander	Central Mexico near Jilotepec	Terrestrial
	A. californiense Gray, 1853	California tiger salamander	Central Valley of California	Terrestrial
	A. cingulatum Cope, 1868	Frosted flatwoods salamander	Southeast United States	Terrestrial
	A. dumerilii	Lake Pátzcuaro salamander, Achoque	West-central Mexico, Lake Pátzcuaro	Neotenic
	A. flavipiperatum Dixon, 1963	Yellow-peppered salamander, Ajolote de Chapala	West-central Mexico	Terrestrial
	A. gracile	Northwestern salamander	Northwest North America	Terrestrial
	A. granulosum Taylor, 1944	Granular salamander, Ajolote	Central Mexico near Toluca	Terrestrial
	A. jeffersonianum	Jefferson salamander	Northeastern North America	Terrestrial
	A. laterale Hallowell, 1856	Blue-spotted salamander	Northeastern North America	Terrestrial
	A. leorae Taylor, 1943	Leora's stream salamander, Ajolote	Central Mexico, Mount Tlaloc	Terrestrial
	A. lermaense	Lake Lerma salamander	Central Mexico, Lake Lerma near Toluca	Terrestrial and neotenic
	A. mabeei Bishop, 1928	Mabee's salamander	Coastal southeast United States	Terrestrial
	A. macrodactylum Baird, 1950	Long-toed salamander	Northwest North America	Terrestrial
	A. maculatum	Spotted salamander	Eastern North America	Terrestrial
	A. mavortium Baird, 1850	Barred tiger salamander	Western North America	Terrestrial and neotenic
	A. mexicanum	Axolotl	Central Mexico, Lake Xochimilco	Neotenic
	A. opacum	Marbled salamander	Eastern United States	Terrestrial
	A. ordinarium Taylor, 1940	Puerto Hondo stream salamander	West-central Mexico, Puerto Hondo stream	Terrestrial and neotenic
	A. rivulare Taylor, 1940	Michoacan stream salamander	Central Mexico	Terrestrial and neotenic
	A. rosaceum Taylor, 1941	Tarahumara salamander	Northwest Mexico, Sierra Madre Occidental	Terrestrial and neotenic
	A. silvense Webb, 2004	Durango salamander	Northwest Mexico, Sierra Madre Occidental	Terrestrial and neotenic
	A. talpoideum Holbrook, 1838	Mole salamander	Southeast United States	Terrestrial and neotenic
	A. taylori Brandon, Maruska, and Rumph, 1982	Taylor's salamander	Southeast Mexico, Laguna Alchichica	Neotenic
	A. texanum Matthes, 1855	Small-mouth salamander	South-central United States	Terrestrial
	A. tigrinum	Eastern tiger salamander	Eastern North America	Terrestrial and neotenic
	A. velasci	Plateau tiger salamander	Mexican Plateau	Terrestrial and neotenic

In addition, two groups of unisexual hybrid populations are sometimes named under their own species: