Radical SAM enzymes


Radical SAM enzymes belong to a superfamily of enzymes that use an iron-sulfur cluster to reductively cleave S-adenosyl-L-methionine to generate a radical, usually a 5′-deoxyadenosyl radical, as a critical intermediate. These enzymes utilize this radical intermediate to perform diverse transformations, often to functionalize unactivated C-H bonds. Radical SAM enzymes are involved in cofactor biosynthesis, enzyme activation, peptide modification, post-transcriptional and post-translational modifications, metalloprotein cluster formation, tRNA modification, lipid metabolism, biosynthesis of antibiotics and natural products etc. The vast majority of known radical SAM enzymes belong to the radical SAM superfamily, and have a cysteine-rich motif that matches or resembles CxxxCxxC. Radical SAM enzymes comprise the largest superfamily of metal-containing enzymes.

History and mechanism

As of 2001, 645 unique radical SAM enzymes have been identified from 126 species in all three domains of life. According to the EFI and SFLD databases, more than 220,000 radical SAM enzymes are predicted to be involved in 85 types of biochemical transformations.
The mechanism for these reactions entail transfer of a methyl or adenosyl group from sulfur to iron. The resulting organoiron complex subsequently releases the organic radical. The latter step is reminiscent of the behavior of adenosyl and methyl cobalamins.

Nomenclature

All enzymes including radical SAM enzymes follow an easy guideline for systematic naming. Systematic naming of enzymes allows a uniform naming process that is recognized by all scientists to understand corresponding function. The first word of the enzyme name often shows the substrate of the enzyme. The position of the reaction on the substrate will also be in the beginning portion of the name. Lastly, the class of the enzyme will be described in the other half of the name which will end in suffix -ase. The class of an enzyme will describe what the enzyme is doing or changing on the substrate. For example, a ligase combines two molecules to form a new bond.
File:RadicalSAMcoredomain front back.png|thumb|Superimposition of three radical SAM core domains. Side views of radical SAM enzymes BioB, MoaA and phTYW1 are shown front and back. This core fold consists of six β/α motifs arranged in a manner that is similar to TIM barrel and is responsible for radical generation. β-sheets are colored yellow and α-helices are shown in cyan. |alt=

Reaction classification

Representative enzymes will be mentioned for each class. Radical SAM enzymes and their mechanisms known before 2008 are summarized by Frey et al. Since 2015, additional review articles on radical SAM enzymes are available, including:
  1. Advances in Radical SAM Enzymology: New Structures and Mechanisms:
  2. Radical S-Adenosylmethionine Enzymes:
  3. Radical S-Adenosylmethionine Enzymes in Cofactor Biosynthesis: A Treasure Trove of Complex Organic Radical Rearrangement Reactions:
  4. Molecular architectures and functions of radical enzymes and their proteins:
  5. Radical SAM enzymes in RiPP biosynthesis.
  6. Radical SAM enzymes with a vitamin B12 -binding domain.

    Carbon methylation

Radical SAM methylases/methyltransferases are one of the largest yet diverse subgroups and are capable of methylating a broad range of unreactive carbon and phosphorus centers. These enzymes are divided into three classes with representative methylation mechanisms. The shared characteristic is the usage of SAM, split into two distinct roles: one as a source of a methyl group donor, and the second as a source of 5'-dAdo radical. Another class has been proposed but proved to be wrongly assigned.

Class A sub-family

  • Class A enzymes methylate specific adenosine residues on rRNA and/or tRNA. In other words, they are RNA base-modifying radical SAM enzymes.
  • The most mechanistically well-characterized are enzymes RlmN and Cfr. Both enzymes methylates substrate by adding a methylene fragment originating from SAM molecule. Therefore, RlmN and Cfr are considered methyl synthases instead of methyltransferases.
File:Radical SAM Enzyme Mmp10.tif|thumb|Structure of a B12-dependent radical SAM enzyme

Class B sub-family

  • Class B enzymes are the largest and most versatile which can methylate a wide range of carbon and phosphorus centers.
  • These enzymes require a cobalamin cofactor as an intermediate methyl group carrier to transfer a methyl group from SAM to substrate.
  • One well-investigated representative enzyme is TsrM which involves in tryptophan methylation in thiostrepton biosynthesis.

    Class C sub-family

  • Class C enzymes are reported to play roles in biosynthesis of complex natural products and secondary metabolites. These enzymes methylate heteroaromatic substrates and are cobalamin-independent.
  • These enzymes contain both the radical SAM motif and exhibit striking sequence similarity to coproporhyrinogen III oxidase, a radical SAM enzyme involved in heme biosynthesis
  • Detailed mechanistic investigations on two class C radical SAM methylases have been reported:
  • # TbtI is involved in the biosynthesis of potent thiopeptide antibiotic thiomuracin.
  • # Jaw5 is suggested to be responsible for cyclopropane modifications.

    Methylthiolation of tRNAs

s belong to a subset of radical SAM enzymes that contain two + clusters and one radical SAM domain. Methylthiotransferases play a major role in catalyzing methylthiolation on tRNA nucleotides or anticodons through a redox mechanism. Thiolation modification is believed to maintain translational efficiency and fidelity.
MiaB and RimO are both well-characterized and bacterial prototypes for tRNA-modifying methylthiotransferases
  • MiaB introduces a methylthio group to the isopentenylated A37 derivatives in the tRNA of S. Typhimurium and E. coli by utilizing one SAM molecule to generate 5'-dAdo radical to activate the substrate and a second SAM to donate a sulfur atom to the substrate.
  • RimO is responsible for post-translational modification of Asp88 of the ribosomal protein S12 in E. coli. The crystal structure sheds light on the mechanistic action of RimO. The enzyme catalyzes pentasulfide bridge formation linking two Fe-S clusters to allow for sulfur insertion to the substrate.
eMtaB is the designated methylthiotransferase in eukaryotic and archaeal cells. eMtaB catalyzes the methylthiolation of tRNA at position 37 on N6-threonylcarbamoyladenosine. A bacterial homologue of eMtaB, YqeV has been reported and suggested to function similarly to MiaB and RimO.

Sulfur insertion into unreactive C-H bonds

Sulfurtransferases are a small subset of radical SAM enzymes. Two well-known examples are BioB and LipA which are independently responsible for biotin synthesis and lipoic acid metabolism, respectively.
  • BioB or biotin synthase is a radical SAM enzyme that employs one center to thiolate dethiobitin, thus converting it to biotin or also known as vitamin B7. Vitamin B7 is a cofactor used in carboxylation, decarboxylation, and transcarboxylation reactions in many organisms.
  • LipA or lipoyl synthase is radical SAM sulfurtransferase utilizing two clusters to catalyze the final step in lipoic acid biosynthesis.

    Carbon insertion

The active site of Mo nitrogenase is the M-cluster, a metal-sulfur cluster containing a carbide at its core. Within the biosynthesis of M-cluster, radical SAM enzyme NifB has been recognized to catalyze a carbon insertion reaction, leading to formation of a Mo/homocitrate-free precursor of M-cluster.

Anaerobic oxidative decarboxylation

  • One well-studied example is HemN. HemN or anaerobic coproporphyrinogen III oxidase is a radical SAM enzyme that catalyzes the oxidative decarboxylation of coproporphyrinogen III to protoporhyrinogen IX, an intermediate in heme biosynthesis. Evidence support the idea that HemN utilizes two SAM molecules to mediate radical-mediated hydrogen transfer for the sequential decarboxylation of the two propionate groups of coproporphyrinogen III.
  • Hyperthermophilic sulfate-reducing archaen Archaeoglobus fulgidus enables anaerobic oxidation of long chain n-alkanes. PflD is reported to be responsible for the capacity of A. fulgidus to grow on a wide range of unsaturated carbons and fatty acids. A detailed biochemical and mechanistic characterization of PflD is still undergoing but preliminary data suggest PflD may be a radical SAM enzyme.

    Protein post-translational modification

  • Formyl-glycine dependent sulfatases require the critical post-translational modification of an active site cysteine or serine residue into a Cα-formylglycine. A radical SAM enzyme called anSME catalyze this post-translational modification in an oxygen-independent manner.

    Protein radical formation

Glycyl radical enzyme activating enzymes are radical SAM subset that can house a stable and catalytically essential glycyl radical in their active state. The underlying chemistry is considered to be the simplest in the radical SAM superfamily with H-atom abstraction by the 5'-dAdo radical being the product of the reaction. A few examples include:
  • Pyruvate formate-lyase activating enzyme catalyzes the activation of PFL, a central enzyme in anaerobic glucose metabolism in microbes.
  • Benzylsuccinate synthase is a central enzyme in anaerobic toluene catabolism.

    Peptide modifications

Radical SAM enzymes that can catalyze sulfur-to-alpha carbon thioether cross-linked peptides generate a class of peptide with antibacterial properties. These peptides belong to the emerging class of ribosomally synthesized and post-translationally modified peptides.
Another subset of peptide-modifying radical SAM enzymes is SPASM/Twitch domain-carrying enzymes. SPASM/Twitch enzymes carry a functionalized C-terminal extension for the binding of two clusters, especially in post-translational modifications of peptides.
The following examples are representative enzymes that can catalyze peptide modifications to generate specific natural products or cofactors.
  1. TsrM in thiostrepton biosynthesis
  2. PoyD and PoyC in polytheonamide biosynthesis
  3. TbtI in thiomuracin biosynthesis
  4. NosN in nosiheptide biosynthesis
  5. EpeE in biosynthesis
  6. MoaA in molybdopterin biosynthesis
  7. PqqE in pyrroloquinoline quinone biosynthesis
  8. TunB in tunicamycin biosynthesis
  9. OxsB in oxetanocin biosynthesis
  10. BchE in anaerobic bacteriochlorophyll biosynthesis
  11. F0 synthases in F420 cofactor biosynthesis
  12. MqnE and MqnC in menaquinone biosynthesis
  13. QhpD in post-translational processing of quinohemoprotein amine dehydrogenase
  14. RumMC2 in ruminococcin C biosynthesis