Anne Amadi was admitted to the bar in Kenya in 1987. After a short stint as State Counsel in the Civil Litigation Department in the Office of the Attorney General, she joined the Judiciary as a District Magistrate in 1991, leaving in 1997 at the rank of Resident Magistrate. She left the Judiciary to practice law privately with her firm, A. A. Amadi & Co. Advocates between 1997 and 2003.
Amadi returned to the Judiciary in January 2014, having been appointed as the Chief Registrar on a five-year contract. She succeeded Gladys Boss Shollei, who left office in October 2013.